Archives of Virology

, Volume 141, Issue 3–4, pp 743–749 | Cite as

Immunisation with DNA polynucleotides protects mice against lethal challenge with St. Louis encephalitis virus

  • R. J. Phillpotts
  • K. Venugopal
  • T. Brooks
Brief Report

Summary

In vivo transfection by intramuscular injection with plasmids expressing the immunogenic proteins of microbial pathogens has considerable potential as a vaccination strategy against many pathogens of both man and animals. Here we report that weanling mice given a single intramuscular injection of 50µg of a plasmid, pSLE1 expressing the St. Louis encephalitis virus (SLE) prM/E protein under the control of the cytomegalovirus immediate early protein promoter produced SLE-specific antibody and were protected against lethal challenge with the virulent virus. Polynucleotide vaccine technology provides a unique opportunity to produce vaccines against flavivirus diseases of low incidence cheaply and rapidly, and to produce multivalent vaccines such as would be required for immunisation against dengue virus disease.

Keywords

Intramuscular Injection Dengue Virus Microbial Pathogen Vaccination Strategy Polynucleotide 

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Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • R. J. Phillpotts
    • 1
  • K. Venugopal
    • 2
  • T. Brooks
    • 1
  1. 1.Microbiology GroupChemical and Biological Defence EstablishmentPorton DownU.K.
  2. 2.Compton LaboratoryAFRC Institute for Animal HealthNewburyU.K.

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