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Archives of Virology

, Volume 141, Issue 3–4, pp 701–704 | Cite as

Full-length genomic sequence of a hepatitis C virus genotype 2c isolate (BEBE1) and the 2c-specific PCR primers

  • H. Nakao
  • H. Okamoto
  • H. Tokita
  • T. Inoue
  • H. Iizuka
  • G. Pozzato
  • S. Mishiro
Brief Report

Summary

We sequenced the entire genome of an Italian isolate of hepatitis C virus: the first full-length sequence for the genotype 2c. We report hereby its characteristics and differential detection of 2c isolates using PCR.

Keywords

Hepatitis Infectious Disease Genomic Sequence Entire Genome Virus Genotype 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Okamoto H, Crovatto M, Pozzato G, Feray C, Brechot C, Mishiro S (1995) Molecular and clinical characteristics of the hepatitis C virus genotype “2c” found in Italians in Italy and France. Int Hepatol Commun 3: 161–165Google Scholar
  2. 2.
    Okamoto H, Sugiyama Y, Okada S, Kurai K, Akahane Y, Sugai Y, Tanaka T, Sato K, Tsuda F, Miyakawa Y, Mayumi M (1992) Typing hepatitis C virus by polymerase chain reaction with type-specific primers: application to clinical surveys and tracing infectious sources. J Gen Virol 73: 673–679PubMedGoogle Scholar
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    Okamoto H, Kobata S, Tokita H, Inoue T, Woodfield GD, Holland PV, AI-Knawy BA, Uzunalimoglu O, Miyakawa Y, Mayumi M (1996) A second-generation method of genotyping hepatitis C virus by the polymerase chain reaction with sense and antisense primers deduced from the core gene. J Virol Methods (in press)Google Scholar
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    Okamoto H, Mishiro S, Tokita H, Tsuda F, Miyakawa Y, Mayumi M (1994) Superinfection of chimpanzees carrying hepatitis C virus of genotype II/1b with that of genotype III/2a or I/1a. Hepatology 20: 1131–1136PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • H. Nakao
    • 1
  • H. Okamoto
    • 1
  • H. Tokita
    • 1
  • T. Inoue
    • 1
  • H. Iizuka
    • 2
  • G. Pozzato
    • 3
  • S. Mishiro
    • 4
  1. 1.Immunology DivisionJichi Medical SchoolTochigiJapan
  2. 2.Japanese Red Cross Saitama Blood CenterSaitamaJapan
  3. 3.Istituto di Medicina ClinicaUniversity of TriesteTriesteItaly
  4. 4.Department of Medical SciencesToshiba General HospitalTokyoJapan

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