Summary
The highly attenuated ‘modified vaccinia Ankara’ (MVA) strain is a potential live vaccine vector. Insertional inactivation of the tk-gene resulted in viruses difficult to purify. Co-integration of a functional fowlpox virus tk-gene allowed easy generation of recombinants, indicating that the genetically stable tk-gene region is a suitable insertion site, if tk-gene activity is substituted.
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Scheiflinger, F., Falkner, F.G. & Dorner, F. Evaluation of the thymidine kinase (tk) locus as an insertion site in the highly attenuated vaccinia MVA strain. Archives of Virology 141, 663–669 (1996). https://doi.org/10.1007/BF01718324
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DOI: https://doi.org/10.1007/BF01718324