Advertisement

Archives of Virology

, Volume 141, Issue 3–4, pp 635–647 | Cite as

Pathogenicity and phylogenetic evaluation of the variant Newcastle disease viruses termed “pigeon PMV-1 viruses” based on the nucleotide sequence of the fusion protein gene

  • M. S. Collins
  • I. Strong
  • D. J. Alexander
Original Papers

Summary

The nucleotide sequences of the entire F genes of two isolates of the pigeon PMV-1 (PPMV-1) variant of Newcastle disease virus (NDV) were determined using RTPCR. The deduced amino acid sequences of the F0 protein showed four differences between isolate 760/83 which had been passaged 4 times in chickens and gave an intravenous pathogenicity index in chickens (IVPI) of 2.01 and isolate 1168/84 which had received six passages in chickens and had an IVPI of 0.00. The F genes of virus from two passage levels of isolate 1447/84, 0 with IVPI value 0.00 and six with IVPI value 0.58, were partially sequenced to cover the areas of variation between 760/83 and 1168/84. The two passage levels of 1447/84 showed identical sequences in these areas which in turn were identical to those of 760/83. It was concluded that the recorded differences in intravenous pathogenicity were unlikely to be associated with differences in the primary structure of the F0 protein. Phylogenetic comparisons of the F gene sequences of the two PPMV-1 viruses with those published for other NDV strains and isolates showed that the PPMV-1 viruses formed a new fourth lineage but were closely related to strain Warwick with which they presumably shared a common origin.

Keywords

Infectious Disease Amino Acid Sequence Nucleotide Sequence Gene Sequence Fusion Protein 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Alexander DJ (1971) Ph.D. Thesis, University of LondonGoogle Scholar
  2. 2.
    Alexander DJ, Parsons, G (1984) Avian paramyxovirus type 1 infections of racing-pigeons: 2. Pathogenicity experiments in pigeons and chickens. Vet Rec 114: 466–469PubMedGoogle Scholar
  3. 3.
    Alexander DJ, Russell PH, Collins MS (1984) Paramyxovirus type 1 infections of racing pigeons: 1. Characterisation of isolated viruses. Vet Rec 114: 444–446PubMedGoogle Scholar
  4. 4.
    Alexander DJ, Russell PH, Parsons G, Abu Elzein EME, Ballouh A, Cernik K, Engstrom B, Fevereiro M, Fleury HJA, Guittet M, Kaleta EF, Kihm U, Kosters J, Lomniczi B, Meister J, Meulemans G, Nerome K, Petek M, Pokomunski S, Polten B, Prip M, Richter R, Saghy E, Samberg Y, Spanoghe L, Tumova B (1985) Antigenic and biological characterisation of avian paramyxovirus type 1 isolates from pigeons — An international collaborative study. Avian Pathol 14: 365–376Google Scholar
  5. 5.
    Alexander DJ, Parsons G (1986) Pathogenicity for chickens of avian paramyovirus type 1 isolates obtained from pigeons in Great Britain during 1983–85. Avian Pathol 15: 487–493Google Scholar
  6. 6.
    Collins MS, Bashiruddin JB, Alexander DJ (1993) Deduced amino acid sequences at the fusion protein cleavage site of Newcastle disease viruses showing variation in antigenicity and pathogenicity. Arch Virol 128: 363–370PubMedGoogle Scholar
  7. 7.
    Collins MS, Strong I, Alexander DJ (1994) Evaluation of the molecular basis of pathogenicity of the variant Newcastle disease viruses termed ‘pigeon PMV-1 viruses’. Arch Virol 134: 403–441PubMedGoogle Scholar
  8. 8.
    Devereux J, Haeberli P, Smithies O (1984) A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res 12: 387–395PubMedGoogle Scholar
  9. 9.
    Felsenstein J (1981) Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17: 368–376PubMedGoogle Scholar
  10. 10.
    Felsenstein J (1985) Confidence limits on phylogenies: an approach using bootstrap. Evolution 39: 783–791Google Scholar
  11. 11.
    Felsenstein J (1988) Phylogenies from molecular sequences: inference and reliability. Annu Rev Genet 22: 521–565PubMedGoogle Scholar
  12. 12.
    Felsenstein J (1991) ‘PHYLIP Manual’, V. 3.4. University Herbarium, University of California, Berkeley, CaliforniaGoogle Scholar
  13. 13.
    Fitch WM (1971) Toward defining the course of evolution: minimum change for a specified tree topology. Syst Zool 20: 406–416Google Scholar
  14. 14.
    Goldman N (1990) Maximum likelihood inference of phylogenetic trees, with special reference to a poisson process model of DNA substitution and to parsimony analyses. Syst Zool 39: 345–361Google Scholar
  15. 15.
    Goldman N, Barton NH (1992) Genetics and geography. Nature 357: 440–441PubMedGoogle Scholar
  16. 16.
    Gotoh B, Sakaguchi T, Nishikawa K, Inocenico NM, Hamaguchi M, Toyoda T, Nagai Y (1988) Structural features unique to each of the three antigenic sites on the haemagglutinin-neuraminidase protein of Newcastle disease virus. Virology 163: 174–182PubMedGoogle Scholar
  17. 17.
    Kimura M (1983) The neutral theory of molecular evolution. Cambridge University Press, London, pp 55–97Google Scholar
  18. 18.
    McGinnes LW, Morrison TG (1987) Nucleotide sequence of the gene encoding the Newcastle disease virus fusion protein and comparisons of paramyxovirus hemagglutinin-neuraminidase protein sequences. Virus Res 7: 187–202PubMedGoogle Scholar
  19. 19.
    McGinnes LW, Morrison TG (1987) The nucleotide sequence of the gene encoding the Newcastle disease virus membrane protein and comparisons of membrane protein sequences. Virus Res 5: 343–356Google Scholar
  20. 20.
    Millar NS, Chambers P, Emmerson PT (1986) Nucleotide sequence analysis of the haemagglutinin-neuraminidase gene of Newcastle disease virus. J Gen Virol 67: 1917–1927PubMedGoogle Scholar
  21. 21.
    Millar NS, Chambers P, Emmerson PT (1986) Nucleotide sequence of the gene encoding the fusion glycoprotein of Newcastle disease virus. J Gen Virol 67: 2685–2694PubMedGoogle Scholar
  22. 22.
    Millar NS, Chambers P, Emmerson PT (1988) Nucleotide sequence of the fusion and haemagglutinin-neuraminidase glycoprotein genes of the Newcastle disease virus, strain Ulster: molecular basis for variations in pathogenicity between strains. J Gen Virol 69: 613–620PubMedGoogle Scholar
  23. 23.
    Miyata T, Miyazawa S, Yasunaga T (1979) Two types of amino acid substitutions in protein evolution. J Mol Evol 12: 219–236PubMedGoogle Scholar
  24. 24.
    Nagai Y, Klenk H-D (1977) Activation of precursors to both glycoproteins of Newcastle disease virus by proteolytic cleavage. Virology 77: 124–134Google Scholar
  25. 25.
    Ohuchi M, Orlich M, Ochuchi R, Simpson BEJ, Garten W, Klenk H-D, Rott R (1989) Mutations at the cleavage site of the hemagglutinin alter the pathogenicity of influenza virus A/Chick/Penn/83(H5N2). Virology 168: 274–280PubMedGoogle Scholar
  26. 26.
    Pritzer E, Kuroda K, Garten W, Nagai Y, Klenk H-D (1990) A host range mutant of Newcastle disease virus with an altered cleavage site for proteolytic activation of the F protein. Virus Res 15: 237–242PubMedGoogle Scholar
  27. 27.
    Rott R, Klenk H-D (1988) Molecular basis of infectivity and pathogenicity of Newcastle disease virus. In: Alexander DJ (ed) Newcastle disease. Kluwer, Norwell, pp 98–112Google Scholar
  28. 28.
    Saitou N, Nei M (1987) The neighbour-joining method: a new method for constructing phylogenetic trees. Mol Biol Evol 4: 406–425PubMedGoogle Scholar
  29. 29.
    Sakaguchi T, Toyoda T, Gotoh B, Inocencio NM, Kuma K, Miyata T, Nagai Y (1989) Newcastle disease virus evolution 1. Multiple lineages defined by sequence variability of the hemagglutinin-neuraminidase gene. Virology 169: 260–272PubMedGoogle Scholar
  30. 30.
    Salminen M, Nykanen A, Brummer-Korvenkontio H, Kantanen ML, Lilitsola K, Leinikki P (1993) Molecular epidemiology of HIV-1 based on phylogenetic analysis of in vivo gag p7/p9 direct sequences. Virology 195: 185–194PubMedGoogle Scholar
  31. 31.
    Sato H, Oh-Hira M, Ishida N, Imamura Y, Hattori S, Kawakita M (1987) Molecular cloning and nucleotide sequence of P, M and F genes of Newcastle disease virus avirulent strain D26. Virus Res 7: 241–255PubMedGoogle Scholar
  32. 32.
    Sato H, Hattori S, Ishida N, Imanura Y, Kawakita M (1987) Nucleotide sequence of the hemagglutinin-neuraminidase gene of Newcastle disease virus avirulent strain D26: evidence for a longer coding region with a carboxy-terminal extension as compared to virulent strains. Virus Res 8: 217–232PubMedGoogle Scholar
  33. 33.
    Schaper UM, Fuller FJ, Ward MDW, Mehrotra Y, Stone HO, Stripp BR, De Buysscher EV (1988) Nucleotide sequence of the envelope protein genes of a highly virulent, neurotropic strain of Newcastle disease virus. Virology 165: 291–295PubMedGoogle Scholar
  34. 34.
    Sneath PHA, Sokal RR (1973) Numerical taxonomy: the principles and practice of numerical classification. WH Freeman, San FranciscoGoogle Scholar
  35. 35.
    Thein SL, Wallace RB (1993) The use of synthetic oligonucleotides as specific hybridization probes in the diagnosis of genetic disorders. In: Davis KE (ed) Human genetic diseases: a practical approach. IRL Press, Herndon, pp 21–33Google Scholar
  36. 36.
    Toyoda T, Sakaguchi T, Imai K, Inocencio NM, Gotoh B, Hamaguchi M, Nagai Y (1987) Structural comparison of the cleavage-activation site of the fusion glycoprotein between virulent and avirulent strains of Newcastle disease virus. Virology 158: 242–247PubMedGoogle Scholar
  37. 37.
    Toyoda T, Sakaguchi T, Hirota H, Gotoh B, Kuma K, Miyata T, Nagai Y (1989) Newcastle disease virus evolution II. Lack of gene recombination in generating virulent and avirulent strains. Virology 169: 273–282PubMedGoogle Scholar
  38. 38.
    Webster RG, Kawaoka Y, Bean WJ (1986) Molecular changes in A/chicken/Pennsylvania/83 (H5N2) influenza virus associated with acquisition of virulence. Virology 148: 165–173Google Scholar

Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • M. S. Collins
    • 1
  • I. Strong
    • 1
  • D. J. Alexander
    • 1
  1. 1.Virology DepartmentCentral Veterinary Laboratory (Weybridge)AddlestoneU.K.

Personalised recommendations