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Archives of Virology

, Volume 141, Issue 3–4, pp 557–572 | Cite as

Retrieval of human cytomegalovirus glycoprotein B from the infected cell surface for virus envelopment

  • K. Radsak
  • M. Eickmann
  • T. Mockenhaupt
  • E. Bogner
  • H. Kern
  • A. Eis-Hübinger
  • M. Reschke
Original Papers

Summary

Surface biotinylation of human cytomegalovirus (HCMV)-infected fibroblasts under pulse-chase conditions was used to define the cellular route of the dominant viral envelope glycoprotein gB into the cytoplasmic compartment of viral maturational envelopment. The results showed that a major fraction of gB was re-internalized from the infected cell surface prior to incorporation into the viral envelope. Viral particles carrying biotinylated gB were subsequently released into the culture medium. Viral release appeared to be inhibited in the presence of gB-specific antibody or when infected cultures were incubated at room temperature, but was not reduced by inhibitors of cellular glycoprotein transport. To our knowledge this is the first report describing that HCMV gB is retrieved from the infected cell surface prior to viral envelopment.

Keywords

Infectious Disease Infected Cell Viral Particle Major Fraction Viral Envelope 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • K. Radsak
    • 1
  • M. Eickmann
    • 1
  • T. Mockenhaupt
    • 1
  • E. Bogner
    • 1
  • H. Kern
    • 2
  • A. Eis-Hübinger
    • 3
  • M. Reschke
    • 1
  1. 1.Institut für VirologieMarburgGermany
  2. 2.Institut für Zellbiologie der Philipps-UniversitätMarburgGermany
  3. 3.Institut für Medizinische Mikrobiologie und ImmunologieBonnGermany

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