Retrieval of human cytomegalovirus glycoprotein B from the infected cell surface for virus envelopment
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Surface biotinylation of human cytomegalovirus (HCMV)-infected fibroblasts under pulse-chase conditions was used to define the cellular route of the dominant viral envelope glycoprotein gB into the cytoplasmic compartment of viral maturational envelopment. The results showed that a major fraction of gB was re-internalized from the infected cell surface prior to incorporation into the viral envelope. Viral particles carrying biotinylated gB were subsequently released into the culture medium. Viral release appeared to be inhibited in the presence of gB-specific antibody or when infected cultures were incubated at room temperature, but was not reduced by inhibitors of cellular glycoprotein transport. To our knowledge this is the first report describing that HCMV gB is retrieved from the infected cell surface prior to viral envelopment.
KeywordsInfectious Disease Infected Cell Viral Particle Major Fraction Viral Envelope
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- 1.Benko D, Gibson W (1989) A major tegument phosphoprotein of human cytomegalovirus contains O-linked GlcNAc. In: Compans RW, Helenius A, Oldstone MB (eds) Cell biology of virus entry, replication and pathogenesis. Alan R. Liss New York, pp 57–66Google Scholar
- 7.Cranage M, Smith G, Bell S, Hart H, Brown C, Bankier A, Tomlinson P, Barrell B, Minson T (1988) Identification and expression of a human cytomegalovirus glycoprotein with homology to the Epstein-Barr virus BXLF2 product, varicella-zoster virus gpIII, and herpes simplex virus type 1 glycoprotein H. J Virol 62: 1416–1422PubMedGoogle Scholar
- 14.Griffiths G, Hoflack B, Simons K, Mellman I, Kornfeld S (1988) The mannose 6-phosphate receptor and the biogenesis of lysosomes. Cell 52: 239–341Google Scholar