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Purification and coat protein gene sequence of a Montana RMV-like isolate of barley yellow dwarf virus

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Summary

A Montana barley yellow dwarf virus (BYDV) isolate, BYDV-RMV-MT, is serologically identical to the New York RMV type isolate (RMV-NY) but differs in aphid transmission phenotype. A purification procedure for BYDV-RMV-MT was developed and cDNAs encompassing the entire coat protein gene and a portion of the putative polymerase gene of both RMV-MT and RMV-NY were cloned and sequenced. Diameters of RMV-MT virions averaged 24.7 nm. Average virus yield was 4.2 mg/kg plant tissue. There was 81% sequence identity between the clones of MT and NY RMV isolates at the nucleotide level. At the amino acid level the polymerase genes were 91% identical to each other and 74% homologous with that of beet western yellow virus. The coat protein amino acid sequences of the two RMV isolates were only 81% identical and, compared to other sequenced luteoviruses, both were most similar to cucurbit aphid-borne yellows virus.

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Authors and Affiliations

  1. Department of Plant Pathology, Montana State University-Bozeman, Bozeman, Montana, USA

    S. M. Geske & T. W. Carroll

  2. USDA-ARS, Department of Plant Pathology, University of Nebraska, Lincoln, Nebraska, USA

    R. French & N. L. Robertson

Authors
  1. S. M. Geske
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  2. R. French
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  3. N. L. Robertson
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  4. T. W. Carroll
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Geske, S.M., French, R., Robertson, N.L. et al. Purification and coat protein gene sequence of a Montana RMV-like isolate of barley yellow dwarf virus. Archives of Virology 141, 541–556 (1996). https://doi.org/10.1007/BF01718316

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  • DOI: https://doi.org/10.1007/BF01718316

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