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The characterization of VP7 (G type) and VP4 (P type) genes of bovine group A rotaviruses from field samples using RT-PCR and RFLP analysis

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Summary

Characterization of the VP7 (G type) and VP4 (P type) genes of bovine group A rotaviruses (BRV) from field samples was performed using RT-PCR and RFLP analysis. After RT-PCR amplification of the full length VP7 genes and partial length VP4 genes (nucleotides 1 to 1096), four enzymes,EcoRV,NlaIV,BamHI andHpaII were used for digestion analysis. For VP7, four RFLP profiles were observed after analysis of the digests: they were designated as G6, G6s (subtype, showed about 86% nucleotide and 90% amino acid identity to reference G6 strains), G8 and G10. For VP4, three RFLP profiles were observed: designated as P[1], P[5] and P[11].

The G typing analysis of 86 BRV fecal samples from 5 states, representing at least 11 different herds revealed that 60.5% (52/86) were G6, which included G6s (9/52); 19.8% (17/86) were G10; 7% (6/86) were G8; 10.4% (9/86) were G6 and G10 mixtures including two G6s samples; and 2.3% (2/86) were G6 and G6s mixtures. The P typing analysis of the same 86 fecal samples revealed that 64% (55/86) were P[5]; 28% (24/86) were P[11]; 1.2% (1/86) were P[1] and 6 samples (7%) were mixtures of either P[11] or P[5]. When the same samples were analyzed according to G and P type specificity, all possible combinations of G and P types existed in the field. The G6P[5] type was most prevalent and accounted for 46.7% (41/86) of the samples; 12.8% (11/86) were G10P[11]; 7% (6/86) were G10P[5] and an equal number were G6sP[11]. The G6P[11] (n=2), G8P[1] (n=1), G8P[5] (n=1) and G8P[11] (n=3) combinations were also observed. The following mixed BRV infections were observed in the field samples; G6sP[5+11] (n=1), G8P[5+11] (n=1), G6+G10P[5] (n=1) G6+G10P[5+11] (n=2), G6+G6sP[11] (n=1), G6+G6sP[1+11] (n=1), G6s+G10P[11] (n=1) and G6s+G10P[5+11] (n=1). Information on the G and P types and G/P combinations in the field samples should be useful for understanding the epidemiology of BRV and designing vaccination strategies to control BRV in the field.

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Authors and Affiliations

  1. Food Animal Health Research Program, Department of Veterinary Prevenitive Medicine, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, Ohio, USA

    K. O. Chang, A. V. Parwani & L. J. Saif

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  1. K. O. Chang
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  2. A. V. Parwani
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  3. L. J. Saif
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Chang, K.O., Parwani, A.V. & Saif, L.J. The characterization of VP7 (G type) and VP4 (P type) genes of bovine group A rotaviruses from field samples using RT-PCR and RFLP analysis. Archives of Virology 141, 1727–1739 (1996). https://doi.org/10.1007/BF01718295

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