Archives of Virology

, Volume 141, Issue 10, pp 2019–2029 | Cite as

Production and characterization of bovine herpesvirus 1 glycoprotein B ectodomain derivatives in an hsp70A gene promoter-based expression system

  • Y. Li
  • S. Van Drunen Littel-Van den Hurk
  • X. Liang
  • L. A. Babiuk
Brief Report

Summary

Different derivatives of bovine herpesvirus 1 (BHV-1) glycoprotein B (gB) ectodomain were expressed in a novel heat-shock expression system. The putative ectodomain, gBt, and the N-terminal subunit, gBb, were of the expected molecular weight and were secreted. Their production were heat-inducible and the purified proteins were able to elicit antibody responses in mice of a comparable level as induced by authentic gB. The truncated C-terminal subunit, gBct, was retained in the endoplasmic reticulum. Our studies suggest that the gBb subunit may play a major role in constituting the overall configuration of gB and is required for the intracellular transport of gB.

Keywords

Molecular Weight Infectious Disease Endoplasmic Reticulum Antibody Response Expression System 

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Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • Y. Li
    • 1
    • 2
  • S. Van Drunen Littel-Van den Hurk
    • 2
  • X. Liang
    • 2
  • L. A. Babiuk
    • 1
    • 2
  1. 1.Department of Veterinary Microbiology, Western College of Veterinary MedicineUniversity of SaskatchewanSaskatoonCanada
  2. 2.The Veterinary Infectious Disease Organization, Western College of Veterinary MedicineUniversity of SaskatchewanSaskatoonCanada

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