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No evidence of mycoplasmas in peripheral blood mononuclear cell fraction of HIV-infected patients

Kein Nachweis von Mykoplasmen in der Fraktion peripherer mononukleärer Blutzellen bei HIV-infizierten Patienten

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Summary

In this study, the prevalence of mycoplasmas in peripheral blood mononuclear cells (PBMC) from HIV-infected individuals was investigated using a mycoplasma genusspecific PCR assay. No mycoplasmas were detected in the PBMC samples from any of the 25 HIV-infected individuals (CDC 2, n=8; CDC 3, n=2; CDC 4, n=15) or ten HIV-seronegative controls. As an internal control, HIV specific sequences were detected in the samples from all HIV-seropositives. These negative results do not support a suggested role of mycoplasmas as co-factor in the progression of HIV infection towards AIDS.

Zusammenfassung

Bei HIV-infizierten Patienten wurde mittels eines Genus-spezifischen PCR-Assay versucht, die Prävalenz von Mykoplasmen in mononukleären Zellen des peripheren Blutes zu bestimmen. In keinem der 25 untersuchten Fälle (CDC-Stadium 2, n=8; Stadium 3, n=2; Stadium 4, n=15) und zehn HIV-seronegativen Kontrollen waren Mykoplasmen in peripheren mononukleären Zellen nachzuweisen. In allen HIV-seropositiven Fällen waren HIV-spezifische Sequenzen mittels PCR nachzuweisen, was als interne Kontrolle dienen kann. Diese negativen Ergebnisse unterstützen die Hypothese nicht, daß Mykoplasmen eine Bedeutung als Kofaktoren der Progression der HIV-Infektion zu AIDS zukommt.

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References

  1. Saillard, C., Carle, P., Bové, J. M., Bébéar, C., Lo, S.-C., Shih, J. W.-K., Wang, R. Y.-H., Rose, D. L., Tully, J. G. Genetic and serologic relatedness betweenMycoplasma fermentans strains and a mycoplasma recently identified in tissues of AIDS and non-AIDS patients. Res. Virol. 141 (1990) 385–395.

    Google Scholar 

  2. Lo, S.-C., Shih, J. W.-K., Yang, N.-Y., Ou, C.-Y., Wang, R. Y.-H. A novel virus-like infectious agent in patients with AIDS. Am. J. Trop. Med. Hyg. 40 (1989) 213–226.

    Google Scholar 

  3. Lo, S.-C., Shih, J. W.-K., Newton III, P. B., Wong, D. M., Hayes, M. M., Benish, J. R., Wear, D. J., Wang, R. Y.-H. Virus-like infectious agent (VLIA) is a novel pathogenic mycoplasma:Mycoplasma incognitus. Am. J. Trop. Med. Hyg. 41 (1989) 586–600.

    Google Scholar 

  4. Bauer, F. A., Wear, D. J., Angritt, P., Lo, S.-C. Mycoplasma fermentans (incognitus strain) infection in the kidneys of patients with acquired immunodeficiency syndrome and associated nephropathy. Human Pathol. 22 (1991) 63–69.

    Google Scholar 

  5. Dawson, M. S., Hayes, M. M., Wang, R. Y.-H., Armstrong, D., Kundsin, R. B., Lo, S.-C. Detection and isolation ofMycoplasma fermentans from urine of human immunodeficiency virus type 1-infected patients. Arch. Pathol. Lab. Med. 117 (1991) 511–514.

    Google Scholar 

  6. Lo, S.-C., Dawson, M. S., Wong, D. M., Newton III, P. B., Sonoda, M. A., Engler, W. F., Wang, R. Y.-H., Shih, J. W.-K., Alter, H. J., Wear, D. J. Identification ofMycoplasma incognitus infection in patients with AIDS: an immunohistochemical,in-situ hybridisation and ultrastructural study. Am. J. Trop. Med. Hyg. 41 (1989) 601–616.

    Google Scholar 

  7. Lo, S.-C., Hayes, M. M., Tully, J. G., Wang, R. Y.-H., Kotani, H., Pierce, P. F., Rose, D. L., Shih, J. W.-K. Mycoplasma penetrans sp. nov., from the urogenital tract of patients with AIDS. Int. J. Syst. Bacteriol. 42 (1992) 357–364.

    Google Scholar 

  8. Montagnier, L., Berneman, D., Guétard, D., Blanchard, A., Chamaret, S., Rame, V., van Rietschoten, J., Mabrouk, K., Bahraoui, E. Inhibition de l'infectiosité de souches prototypes du VIH par des anticorps dirigés contre une sequence peptidique de mycoplasma. C. R. Acad. Sci. Paris 311 (1990) 425–430.

    Google Scholar 

  9. Phillips, D. M., Pearce-Pratt, R., Tan, X., Zacharopoulos, V. R. Association of mycoplasma with HIV-1 and HTLV-I in human T lymphocytes. AIDS Res. Hum. Retroviruses 8 (1992) 1863–1868.

    Google Scholar 

  10. Franzoso, G., Dimitrov, D. S., Blumenthal, R., Barile, M. F., Rottem, S. Fusion ofMycoplasma fermentans strain incognitus with T-lymphocytes. FEBS Letters 303 (1992) 251–254.

    Google Scholar 

  11. Chowdhury, M. I. H., Koyanaga, Y., Kobayashi, S., Yamamoto, N., Munakata, T., Arai, S. Mycoplasma and AIDS. Lancet 336 (1990) 247–248.

    Google Scholar 

  12. Lemaitre, M., Henin, Y., Destouesse, F., Ferrieux, C., Montagnier, L., Blanchard, A. Role of mycoplasma infection in the cytopathic effect induced by human immunodeficiency virus type 1 in infected cell lines. Infect. Immun. 60 (1992) 742–748.

    Google Scholar 

  13. Lo, S.-C., Tsai, S., Benish, J. R., Shih, J. W.-K., Wear, D. J., Wong, D. M. Enhancement of HIV-1 cytocidal effects in CD4 lymphocytes by the AIDS-associated mycoplasma. Science. 251 (1991) 1074–1075.

    Google Scholar 

  14. Lemaitre, M., Guétard, D., Hénin, Y., Montagnier, L., Zerial, A. Protective activity of tetracycline analogs against the cytopathic effect of the human immunodeficiency viruses in CEM cell. Res. Virol. 141 (1990) 5–16.

    Google Scholar 

  15. van Kuppeveld, F. J. M., van der Logt, J. T. M., Angulo, A. F., van Zoest, M. J., Quint, W. G. V., Niesters, H. G. M., Galama, J. M. D., Melchers, W. J. G. Genus- and species-specific identification of mycoplasmas by 16S rRNA amplification. Appl. Environ. Microbiol. 58 (1992) 2606–2615. Author's correction, 59 (1993) 655.

    Google Scholar 

  16. Boyum, A. Separation of leucocytes from blood and bone marrow. Scand. J. Clin. Lab. Invest. 21 (Suppl. 97) (1968) 1–5.

    Google Scholar 

  17. Maniatis, T., Fritsch, E. F., Sambrook, J. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold spring Harbor, N. Y. (1980).

    Google Scholar 

  18. van Kuppeveld, F. J. M., Johansson, K.-E., Galama, J. M. D., Kissing, J., Bölske, G., van der Logt, J. T. M., Melchers, W. J. G. Detection of mycoplasma contamination in cell cultures by a mycoplasma group-specific PCR. Appl. Environ. Microbiol. 60 (1994) 149–152.

    Google Scholar 

  19. Hawkins, R. E., Rickman, L. S., Vermund, S. H., Carl, M. Association of mycoplasma and human immunodeficiency virus infection: detection of amplifiedMycoplasma fermentans DNA in blood. J. Infect. Dis. 165 (1992) 581–585.

    Google Scholar 

  20. Katseni, V. L., Gilroy, C. B., Ryait, B. K., Ariyoshi, K., Bieniasz, P. D., Weber, J. N., Taylor-Robinson, D. Mycoplasma fermentans in individuals seropositive and seronegative for HIV-1. Lancet 341 (1993) 271–273.

    Google Scholar 

  21. Bebear, C., de Barbeyrac, B., Clere, M.-T., Renaudin, H., Fleury, H. J. A., Dupon, M., Ragnaud, J.-M., Morlat, P. Mycoplasmas in HIV-1 seropositive patients. Lancet 341 (1993) 758–759.

    Google Scholar 

  22. Wang, R. Y.-H., Shih, J. W.-K., Grandinetti, T., Pierce, P. F., Hayes, M. M., Wear, D. J., Alter, H. J., Lo, S.-C. High frequency of antibodies toMycoplasma penetrans in HIV-infected patients. Lancet 340 (1992) 1312–1316.

    Google Scholar 

  23. Wang, R. Y.-H., Shih, J. W.-K., Weiss, S. H., Grandinetti, T., Pierce, P. F., Lange, M., Alter, H. J., Wear, D. J., Davies, C. L., Mayur, R. K., Lo, S.-C. Mycoplasma penetrans infection in male homosexuals with AIDS: high seroprevalence and association with Kaposi's sarcoma. Clin. Infect. Dis. 17 (1993) 724–729.

    Google Scholar 

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Authors and Affiliations

  1. Dept. of Microbiology, University of Nijmegen, P. O. Box 9101, NL-6500 HB, Nijmegen, The Netherlands

    F. van Kuppeveld M.Sc., W. Melchers Ph.D., J. Kissing B.Sc., J. van der Logt Ph.D. & J. Galama M.D., Ph.D.

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  1. F. van Kuppeveld M.Sc.
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  2. W. Melchers Ph.D.
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  3. J. Kissing B.Sc.
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  4. J. van der Logt Ph.D.
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  5. J. Galama M.D., Ph.D.
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van Kuppeveld, F., Melchers, W., Kissing, J. et al. No evidence of mycoplasmas in peripheral blood mononuclear cell fraction of HIV-infected patients. Infection 22, 204–206 (1994). https://doi.org/10.1007/BF01716704

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  • DOI: https://doi.org/10.1007/BF01716704

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