Summary
This study characterized acute peritonitis and chronic abscess formation resulting from experimental mixed anaerobic infection withBacteroides melaninogenicus andFusobacterium necrophorum. At intervals after infection liver and spleen samples were obtained, fixed, and processed for histological examination. An acute to chronic infection progressed in mice infected with this mixture of anaerobic bacteria, whereas, no infection resulted when either organism was injected alone. Acute inflammatory cell infiltrates were noted in tissue samples at 12 h postinfection. Small, discrete areas of liver cell necrosis with neutrophilic infiltrates were observed as early as 24 h. By 48 h after infection the liver parenchyma was infiltrated with both acute and chronic inflammatory cells, with moderate to severe hepatocyte degeneration recognized at 72 h. Large intrahepatic abscesses were present in the subphrenic (upper lobe) area 2 to 6 weeks after experimental infection.
Similar content being viewed by others
References
Onderdonk AB, Kasper DL, Mansheim BJ, Louie TJ, Gorbach SL, Bartlett JG (1979) Experimental animal models for anaerobic infections. Rev Infect Dis 1:291–301
Wilkins TD, Walker CB, Nitzan D, Salyers AA (1977) Experimental infection with anaerobic bacteria in mice. J Infect Dis (Suppl) 135:S13-S17
Hill GB, Osterhout S, Pratt PC (1974) Liver abscess production by non-sporeforming anaerobic bacteria in a mouse model. Infect Immun 9:599–603
Nichols RL (1980) Infection following gastrointestinal surgery: Intraabdominal abscess. Surg Clin N Am 60:197–211
McCallum RE, Urbaschek R, Urbaschek B (1982) Host response to mixed anaerobic infection withBacteroides melaninogenicus andFusobacterium necrophorum. Infection (in press)
Brown JH, Brenn L (1931) A method for differential staining of Gram positive and Gram negative bacteria in tissue sections. Bull Johns Hopkins Hosp 48:69–73
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
McCallum, R.E., Rohrer, M.D., Urbaschek, R. et al. Histopathology of mixed anaerobic intraabdominal infection in mice. Klin Wochenschr 60, 702–704 (1982). https://doi.org/10.1007/BF01716558
Issue Date:
DOI: https://doi.org/10.1007/BF01716558