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Structure and properties of TentaGel resin beads: Implications for combinatorial library chemistry

Molecular Diversity volume 1, pages 223–232 (1996)Cite this article

Summary

In view of the widespread use of TentaGel resin beads for the synthesis of combinatorial libraries, the properties of TentaGel resin have been examined using a combination of confocal laser microscopy and NMR spectroscopy. Evidence is presented that trypsin, a 23.5-kDa enzyme, can penetrate to the core of 90-μm TentaGel beads, and that the matrix of such beads permits molecular motion at a similar rate to that in solution. The beads act as a separate gel phase rather than as a porous solid. These conclusions have important implications for the bioassay of on-bead combinatorial chemical libraries.

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Authors and Affiliations

  1. Dyson Perrins Laboratory, Oxford University, South Parks Road, OX1 3QY, Oxford, UK

    Rachel Quarrell, Timothy D. W. Claridge, George W. Weaver & Gordon Lowe

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  1. Rachel Quarrell
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  2. Timothy D. W. Claridge
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  3. George W. Weaver
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  4. Gordon Lowe
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Quarrell, R., Claridge, T.D.W., Weaver, G.W. et al. Structure and properties of TentaGel resin beads: Implications for combinatorial library chemistry. Mol Divers 1, 223–232 (1996). https://doi.org/10.1007/BF01715526

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  • DOI: https://doi.org/10.1007/BF01715526

Keywords

  • Combinatorial libraries
  • TentaGel resin beads
  • 31P NMR
  • Spin-lattice relaxation
  • Trypsin
  • Confocal scanning laser microscope