Zusammenfassung
Mit einer als klinischer Routinemethode brauchbaren Modifikation des vonSterling u.Brenner [14] angegebenen Verfahrens unter Verwendung von125J-markiertem Thyroxin wurden normale Mittelwerte von 0,026±0,005% bzw. 2,22±0,69 mµg-% für das freie Thyroxin im Serum ermittelt. Bei Hyperthyreose ist das freie Thyroxin (%) stark erhöht, bei Gravidität gesenkt.
Summary
Application of a modified Sterling-Brenner [14]-procedure using125I-labelled thyroxine as tracer substance, resulted in a normal mean value of 0.026±0.005% resp. 2.22±0.69 mµg-% free thyroxine in human serum. In hyperthyroidism free thyroxine (%) in serum is increased, in pregnancy decreased.
Literatur
Bellabarba, D., R. E. Peterson, andK. Sterling: An improved method for chromatography of iodothyronines. J. clin. Endocr.28, 305 (1968).
Emrich, D., H. Reichenbach, G. Hoffmann u.W. Keiderling: Vergleichende papierchromatographische Untersuchungen von131J-markierten Jodaminosäuren. Nucl.-Med. (Stuttg.)6, 149 (1967).
Gleason, G. J.: Some notes on the exchange of iodine with thyroxine homologues. J. biol. Chem.213, 873 (1955).
Gmelin, R., andA. J. Virtanen: A sensitive colour reaction for the paperchromatographic detection of iodide, iodinated tyrosines and thyronines. Acta chem. scand.13, 1469 (1959).
Hollander, D., J. C. Meek, andR. T. Manning: Determination of free thyroxine in serum of patients with cirrhosis of the liver. New Engl. J. Med.276, 900 (1967).
Ingbar, S. H., L. E. Braverman, N. A. Dawber, andJ. Y. Lee: A new method for measuring the free thyroid hormone in human serum and an analysis of the factors that influence its concentration. J. clin. Invest.44, 1679 (1965).
Lamberg, B. A., andK. Liewendahl: Free thyroxine in serum. Scand. J. clin. Lab. Invest.19, Suppl. 100, 115 (1967).
Lardy, H. in: The thyroid, Brookhaven Symp. Biol.7, 94 (1955).
Murphy, B. E. P., andC. E. Pattee: Determination of thyroxine utilizing the property of protein-binding. J. clin. Endocr.24, 187 (1964).
Oppenheimer, J. H., andM. J. Surks: Determination of free thyroxine in human serum; a theoretical and experimental analysis. J. clin. Endocr.24, 785 (1964).
Recant, L., andD. S. Riggs: Thyroid function in nephrosis. J. clin. Invest.31, 789 (1952).
Robbins, J., andJ. E. Rall: The interaction of thyroid hormones and protein in biological fluids. Recent Progr. Horm. Res.13, 161 (1957).
Schussler, G. C., andJ. E. Plager: Effect of preliminary purification of131J-thyroxine on the determination of free thyroxine in serum. J. clin. Endocr.27, 242 (1967).
Sterling, K., andM. A. Brenner: Free thyroxine in human serum: simplified measurement with aid of magnesium precipitation. J. clin. Invest.45, 153 (1966).
Sterling, K., andA. Hegedus: Measurement of free thyroxine concentration in human serum. J. clin. Invest.41, 1031 (1962).
Volpert, E. M., M. Martinez, andJ. H. Oppenheimer: Radioiodinated impurities in commercial preparations of131J-thyroxine and their effect on the measurement of free thyroxine in human serum by equilibrium dialysis. J. clin. Endocr.27, 421 (1967).
Zappi, E., andG. Bublitz: Combined chemical and isotopic detection of iodoamino acids in thin layer chromatography. J. Chromatogr.35, 441 (1968).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Herrmann, J., Krüskemper, H.L. Bestimmung des freien Thyroxins im Serum mit125J-markiertem Thyroxin. Klin Wochenschr 46, 1285–1286 (1968). https://doi.org/10.1007/BF01711878
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01711878