Abstract
The rate of recovery and time to detection of mycobacteria from clinical specimens were analysed for specimens inoculated into both radiometric Middlebrook 7H12 (Bactec 12B, originally 12A) medium and Lowenstein-Jensen egg-based medium (with and without sodium pyruvate) over the 15-year period from 1980 to 1994. A total of 19,679 Bactec vials were inoculated together with Lowenstein-Jensen slopes, with 2198 mycobacterial isolates detected. The mean times to detection for Bactec and Lowenstein-Jensen were 11.7 days and 23.9 days, respectively. In 195 cases mycobacteria were isolated from Bactec and not from Lowenstein-Jensen, whereas the reverse was true in 42 cases. The number of contaminated Bactec vials was 488 and the number of contaminated Lowenstein-Jensen slopes 448.
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References
Siddiqi SH, Libonati JP, Middlebrook G: Evaluation of a rapid radiometric method for drug susceptibility testing ofMycobacterium tuberculosis. Journal of Clinical Microbiology 1981, 13: 908–912.
Chiyoji A, Hosojima S, Fukasawa Y, Kazumi Y, Takahashi M, Hirano K, Mori T: Comparison of MB-Check, Bactec and egg-based media for recovery of mycobacteria. Journal of Clinical Microbiology 1992, 30: 878–881.
Damato JJ, Collins MT, Rothlauf MV, McClathy JK: Detection of mycobacteria by radiometric and standard plate procedures. Journal of Clinical Microbiology 1993, 17: 1066–1073.
Morgan MA, Horstmeier CD, DeYoung DR, Roberts GD: Comparison of a radiometric method (Bactec) and conventional culture media for recovery of mycobacteria from smear-negative specimens. Journal of Clinical Microbiology 1983, 18: 384–388.
Sewell DL, Rashad AL, Rourke WJ, Poor SL, McCarthy JC, Pfaller MA: Comparison of the Septi-Chek AFB and Bactec systems and conventional culture for recovery of mycobacteria. Journal of Clinical Microbiology 1993, 31: 2689–2691.
Wilson ML, Stone BL, Hildred MV, Reves RR: Comparison of recovery rates for mycobacteria from Bactec 12B vials, Middlebrook 7H11-Selective 7H11 Biplates and Lowenstein-Jensen slants in a public health mycobacteriology laboratory. Journal of Clinical Microbiology 1995, 33: 2516–2518.
Stager CE, Libonati JP, Siddiqi SH, Davis JR, Hooper NM, Baker JF, Carter ME: Role of solid media when used in conjunction with the Bactec system for mycobacterial isolation and identification. Journal of Clinical Microbiology 1991, 29: 154–157.
Takahashi H, Foster V: Detection and recovery of mycobacteria by a radiometric procedure. Journal of Clinical Microbiology 1983, 17: 380–381.
DeLand FH, Wagner HN Jr: Early detection of bacterial growth with carbon-14 labelled glucose. Radiology 1969, 92: 154–155.
Cummings DM, Ristroph D, Camargo EF, Larson SM, Wagner HN Jr: Radiometric detection of the metabolic activity ofMycobacterium tuberculosis. Journal of Nuclear Medicine 1975, 16: 1189–1191.
Allen BW: Inhibitory effect of rubber on the growth ofM. tuberculosis. Tubercle 1980, 61: 91–95.
Chadwick MV: Mycobacteria. Wright PSG, Bristol, UK, 1982, p. 45.
Brooks RW, George KL, Parker BC, Falkinham JO III: Recovery and survival of non-tuberculous mycobacteria under various growth and decontamination conditions. Canadian Journal of Microbiology 1984, 30: 1112.
Roberts GD, Goodman NL, Heifets L, Larsh HW, Lindner TH, McClatchy K, McGinnis MR, Siddiqi SH, Wright P: Evaluation of the Bactec radiometric method for recovery of mycobacteria and drug susceptibility testing ofMycobacterium tuberculosis from acid fast smear positive specimens. Journal of Clinical Microbiology 1983, 18: 689–696.
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Middleton, A.M., Chadwick, M.V. & Gaya, H. Evaluation of a commercial radiometric system for primary isolation of mycobacteria over a fifteen-year period. Eur. J. Clin. Microbiol. Infect. Dis. 16, 166–170 (1997). https://doi.org/10.1007/BF01709480
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DOI: https://doi.org/10.1007/BF01709480