Abstract
A polymerase chain reaction (PCR) assay was applied for the selective amplification of a characteristic sequence within aSalmonella-specific chromosomal fragment. A two-temperature PCR cycle enhanced both the speed and overall sensitivity of the amplification procedure. Twenty-one well-characterizedSalmonella strains and a number of non-Salmonella strains were tested. With the exception of the rarely isolatedSalmonella arizonae strain, the PCR-based approach enabled the specific identification ofSalmonella with a detection limit of 103 organisms. In combination with a nested PCR assay, as few as ten organisms were detectable. Specificity was demonstrated as no distinct amplification products were detectable with any of the testednon-Salmonella strains. With a pre-enrichment step using paramagnetic antiSalmonella beads, an increase in sensitivity was observed in the case of clinical samples while the amplification process was not influenced.
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Haedicke, W., Wolf, H., Ehret, W. et al. Specific and sensitive two-step polymerase chain reaction assay for the detection ofSalmonella species. Eur. J. Clin. Microbiol. Infect. Dis. 15, 603–607 (1996). https://doi.org/10.1007/BF01709372
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DOI: https://doi.org/10.1007/BF01709372