Abstract
Thirty healthy blood donors, 15 workers from horse-breeding farms, 69 human immunodeficiency virus (HIV)-negative persons at risk for HIV infection, 125 HIV-infected subjects withoutRhodococcus equi infection, and nine HIV-infected patients withRhodococcus equi pneumonia were evaluated in order to detect serum antibodies toRhodococcus equi precipitate-soluble antigen by an enzyme immunoassay (EIA). Whereas EIA values for healthy donors, horse farm workers, individuals at risk for HIV infection, and HIV-positive subjects withoutRhodococcus equi infection were comparable, HIV-infected patients with rhodococcal disease had significantly higherRhodococcus equi antibody levels (p<0.0001). The clinical outcome ofRhodococcus equi pneumonia was more severe in subjects who had low levels of specific antibodies, whereas patients who recovered had elevatedRhodococcus equi antibody levels over time. Immunoblot studies showed that bothRhodococcus equi-infected patients and foals recognized a protein band of approximately 60 kDa in theRhodococcus equi precipitate-soluble antigen. On the other hand, theRhodococcus equi-infected patients did not react with the diffuse 15 to 17 kDa virulence-associated proteins that represent important virulence factors both in mice and horses.
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Prescott JF:Rhodococcus equi: an animal and human pathogen. Clinical Microbiology Reviews 1991, 4: 20–34.
Nordmann R Ronco E, Nauciel C: Role of T-lymphocyte subsets inRhodococcus equi infection. Infection and Immunity 1992, 60: 2748–2752.
Woolcock JB, Mutimer MD, Bowles PM: The immunological response of foals toRhodococcus equi: a review. Veterinary Microbiology 1987, 14: 215–224.
Takai S, Kawazu S, Tusbaki S: Humoral immune response of foals to experimental infection withRhodococcus equi. Veterinary Microbiology 1987, 14: 321–327.
Takai S, Kawazu S, Tsubaki S: Immunoglobulin and specific antibody responses toRhodococcus (Corynebacterium) equi infection in foals as measured by enzymelinked immunosorbent assay. Journal of Clinical Microbiology 1986, 23: 943–947.
Takai S, Koike K, Ohbushi S, Izumi C, Tsubaki S: Identification of 15- to 17-kilodalton antigens associated with virulentRhodococcus equi. Journal of Clinical Microbiology 1991, 29: 439–443.
Linder R, Bernheimer AW: Enzymatic oxidation of membrane cholesterol in relation to lysis of sheep erythrocytes by corynebacterial enzymes. Archives of Biochemistry and Biophysics 1982, 213: 395–404.
Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227: 680–685.
Mastroianni CM, Lichtner M, Mengoni F, Mascellino MT, Vullo V, Delia S:Rhodococcus equi virulence-associated antigens and specific antibody response in AIDS patients infected withRhodococcus equi. Clinical Microbiology and Infection 1995, 1: 18–23.
Machang'u RS, Prescott JF: Role of antibody to extracellular proteins ofRhodococcus equi in protection againstR. equi pneumonia in foals. Veterinary Microbiology 1991, 26: 323–333.
Machang'u RS, Prescott JF: Purification and properties of cholesterol oxidase and choline phosphohydrolase fromRhodococcus equi. Canadian Journal of Veterinary Research 1991, 55: 332–340.
Takai S, Sekizaki T, Ozawa T, Suguwara T, Watanabe Y, Tsubaki S: Association between a large plasmid and 15- to 17-kilodalton antigens in virulentRhodococcus equi. Infection and Immunity 1991, 59: 4056–4060.
Tan C, Prescott JF, Patterson MC, Nicholson VM: Molecular characterization of a lipid-modified virulence-as-sociated protein ofRhodococcus equi and its potential in protective immunity. Canadian Journal of Veterinary Research 1995, 59: 51–59.
Takai S, Sasaki Y, Ikeda T, Uchida Y, Tsubaki S, Sekizaki T: Virulence ofRhodococcus equi isolates from patients with and without AIDS. Journal of Clinical Microbiology 1994, 32: 457–460.
Mutimer MD, Woolcock JB, Sturgess BR:Corynebacterium equi in human faeces. Australian Medical Journal 1979, 2: 422.
Verville TD, Huycke MM, Greenfield RA, Fine DP, Kuhls TL, Slater LN:Rhodococcus equi infections in humans. 12 cases and review of the literature. Medicine 1994, 73: 119–132.
Lane HC, Masur H, Edgar LC, Whalen G, Rook AH, Fauci AS: Abnormalities of B-cell activation and immunoregulation in patients with the acquired immunodeficiency syndrome. New England Journal of Medicine 1983, 309: 453–458.
Amman AJ, Schiffman G, Abrams D, Volberding P, Ziegler J, Conant M: B-cell immunodeficiency in acquired immunodeficiency syndrome. Journal of the American Medical Association 1984, 251: 1447–1449.
Peglow SL, Smulian G, Linke MJ, Pogue CL, Nurre S, Crisler J, Phair J, Gold JWM, Armstrong D, Walzer PD: Serologic responses toPneumocystis carinii antigens in health and disease. Journal of Infectious Diseases 1990, 161: 296–306.
Lundgren B, Lundgren JD, Nielsen T, Mathiesen L, Nielsen JO, Kovacs JA: Antibody response to a majorPneumocystis carinii antigen in human immunodeficiency virus-infected patients with and withoutP. carinii pneumonia. Journal of Infectious Diseases 1992, 165: 1151–1155.
Hietala SK, Ardans AA, Sansome A: Detection ofCorynebacterium equi-specific antibody in horses by enzyme-linked immunosorbent assay. American Journal of Veterinary Research 1985, 46: 13–15.
Martens RJ, Martens JG, Fiske RA, Hietala SK:Rhodococcus equi foal pneumonia: protective effects of immune plasma in experimentally infected foals. Equine Veterinary Journal 1989, 21: 249–255.
Mastroianni CM, Lichtner M, Vullo V, Delia S: Humoral immune response toRhodococcus equi in AIDS patients withR. equi pneumonia. Journal of Infectious Diseases 1994, 169: 1179–1180.
Prescott JF, Coshan-Gauthier R, Barksdale L: Antibody to equi factor(s) in the diagnosis ofCorynebacterium equi pneumonia in foals. Canadian Journal of Comparative Medicine 1984, 48: 370–373.
Skalka B: Dynamics of equi-factor antibodies in sera of foals kept on farms with differing histories ofRhodococcus equi pneumonia. Veterinary Microbiology 1987, 14: 269–276.
Takai S, Madarame H, Matsumoto C, Inoue M, Sasaki Y, Hasegawa Y, Tsubaki S, Nakane A: Pathogenesis ofRhodococcus equi infection in mice: roles of virulence plasmids and granulomagenic activity of bacteria. FEMS Immunology and Medical Microbiology 1995, 11: 181–190.
Takai S, Imai Y, Fukunaga N, Uchida Y, Kamisawa K, Sasaki Y, Tsubaki S, Sekizaki T: Identification of virulence-associated antigens and plasmids inRhodococcus equi from patients with AIDS. Journal of Infectious Diseases 1995, 172: 1306–1311.
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Vullo, V., Mastroianni, C.M., Lichtner, M. et al. Serologic responses toRhodococcus equi in individuals with and without human immunodeficiency virus infection. Eur. J. Clin. Microbiol. Infect. Dis. 15, 588–594 (1996). https://doi.org/10.1007/BF01709368
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DOI: https://doi.org/10.1007/BF01709368