Abstract
Listeria monocytogenes was isolated from three patients hospitalized in two departments of the same hospital over a two-week period. A nosocomial outbreak of listeriosis was suspected. Patients presented with tenosynovitis, central venous catheter infection, and bacteremia. The first patient had a community-acquired infection, the second a nosocomial infection, and the source of the third patient's illness was uncertain. Epidemiological investigations failed to identify a common source of contamination within the hospital. The three strains were nontypeable by phage typing, but Smal macrorestriction analysis and pulsed-field gel electrophoresis yielded three distinct profiles. Therefore, the three cases seemed to represent a cluster of sporadic cases as opposed to an outbreak of listeriosis. Rapid typing of isolates is essential in the early investigation of potential outbreaks of listeriosis and may prevent the initiation of expensive and time-consuming epidemiological investigations.
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Jacquet C, Michelon F, Saint-Cloment C, Rocourt J: La listériose humaine en France en 1994, données du Centre National de Référence desListeria. Bulletin Epidémiologique Hebdomadaire 1995, 39: 173–174.
Schuchat A, Swaminathan B, Broome CV: Epidemiology of human listeriosis. Clinical Microbiology Reviews 1991, 4: 169–183.
Seeliger HPR, Hohne K: Serotyping ofL. monocytogenes and related species. In: Bergan T, Norris JR (ed): Methods in microbiology. Academic Press, New York, 1979, p. 31–49.
Loesser MJ, Busse M: Bacteriophage typing ofListeria species. Applied and Environmental Microbiology 1990, 56: 1912–1918.
Graves LM, Swaminathan B, Reeves MW, Wenger J: Ribosomal DNA fingerprinting ofListeria monocytogenes using a digoxigenin-labelled DNA probe. European Journal of Epidemiology 1991, 7: 77–82.
Selander RK, Caugant DA, Ochman H, Musser JM, Gilmour MN, Wittman T: Methods of multilocus enzyme electrophoresis for bacterial population genetics and systematics. Applied and Environmental Microbiology 1986, 51:873–884.
Ridley AM: Evaluation of a restriction fragment length polymorphism typing method forListeria monocytogenes. Research in Microbiology 1995, 146: 21–34.
Brosch R, Buchrieser C, Rocourt J: Subtyping ofListeria monocytogenes serovar 4b by use of low-frequency-cleavage restriction endonuclease and pulsed-field gel electrophoresis. Research in Microbiology 1991, 142: 667–675.
Mazurier SI, Audurier A, Marquet-Van der Mee N, Notermans S, Wernars K: A comparative study of randomly amplified polymorphic DNA analysis and conventional phage typing for epidemiological studies ofListeria monocytogenes isolates. Research in Microbiology 1992, 143:507–512.
Swaminathan B, Rocourt J, Bille J:Listeria. In: Murray PR, Baron EJ, Pfaller MA, Tenover FG, Yolken RH (ed): Manual of clinical microbiology. American Society for Microbiology, Washington DC, 1995, p. 341–348.
Rocourt J, Audurier A, Courtieu AL, Dust J, Ortel S, Schrettenbrunner A, Taylor AG: A multi-centre study on the phage typing ofListeria monocytogenes. Zentralblatt für Bakteriologie, Mikrobiologie, und Hygiene [A] 1985, 259: 489–497.
Morrell RM, Wasilauskas B: Tracking laboratory contamination by using aBacillus cereus pseudoepidemic as an example. Journal of Clinical Microbiology 1992, 30: 1469–1473.
Jacquet C, Catimel B, Brosch R, Buchrieser C, Dehaumont P, Goulet V, Lepoutre A, Veit P, Rocourt J: Investigations related to the epidemic strain involved in the French listeriosis outbreak. Applied and Environmental Microbiology 1992, 61: 2242–2246.
Schwartz B, Hexter D, Broome CV, Hightower AW, Hirschorn RB, Porter JD, Hayes PS, Bibb WF, Lorber B, Faris DG: Investigation of an outbreak of listeriosis: new hypotheses for the etiology of epidemicListeria monocytogenes infections. Journal of Infectious Diseases 1989, 159:680–685.
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La Scola, B., Fournier, P.E., Musso, D. et al. Pseudo-outbreak of listeriosis elucidated by pulsed-field gel electrophoresis. Eur. J. Clin. Microbiol. Infect. Dis. 16, 756–760 (1997). https://doi.org/10.1007/BF01709260
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DOI: https://doi.org/10.1007/BF01709260