Abstract
The performance of a commercially available assay for detection of hepatitis C virus (HCV) antibody in saliva samples was assessed. Samples of saliva were collected from 270 individuals whose HCV antibody status was determined by serum assay (161 HCV-positive, 109 HCV-negative). The saliva samples were tested for the presence of HCV antibodies using a modified protocol. The sensitivity was 94.4% (95% CI, 89.3–97.2%) and the specificity 99.1% (95% CI, 94.3–100%). Although the optical density in tests on HIV-positive individuals was lower than that among HIV-negative individuals, the HIV status had no significant influence on the results of the HCV assay in saliva. These findings suggest that tests on saliva can be useful in epidemiological studies for estimating the prevalence of HCV in populations that are difficult to reach.
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References
Conrycantilena C: Hepatitis C virus diagnostics — technology, clinical applications and impacts. Trends in Biotechnology (1997) 15:71–76
Frerichs RR, Silarug N, Eskes N, Pagcharoenpol P, Rodklai A, Thangsupachai S, Wongba C: Saliva-based HIV-antibody testing in Thailand. AIDS (1994) 8:885–894
Noone A, Durante AJ, Brady AR, Majid F, Swan AV, Parry JV, Hart GJ, Connell JA, Perry KR, Joce RE, Gill NO: HIV infection in injecting drug users attending centres in England and Wales, 1990–1991. AIDS (1993) 7:1501–1507
Connell JA, Parry JV, Mortimer PP, Duncan J: Novel assay for the detection of immunoglobulin G antihuman immunodeficiency virus in untreated saliva and urine. Journal of Medical Virology (1993) 41:159–164
Sherman KE, Creager RL, O'Brien J, Sargent S, Piacentini S, Thieme T: The use of oral fluid for hepatitis C antibody screening. American Journal of Gastroenterology (1994) 89:2025–2027
Fleiss JF: Statistical methods for rates and proportions. John Wiley and Sons, New York (1981): p. 321
Thieme T, Yoshihara P, Piacentini S, Beller M: Clinical evaluation of oral fluid samples for diagnosis of viral hepatitis. Journal of Clinical Microbiology (1992) 30:1076–1079
Mcintyre PG, Laszlo J, Appleyard K, Ogden GR: Modified enzyme immunoassay to detect hepatitis C virus antibodies in oral fluid. European Journal of Clinical Microbiology & Infectious Diseases (1996) 15:882–884
Emmons WW, Paparello SF, Decker CF, Sheffield JM, Lowe Bey FH: A modified ELISA and Western blot accurately determine anti-human immunodeficiency virus type 1 antibodies in oral fluids obtained with a special collecting device, Journal of Infectious Diseases (1995) 171:1406–1410
Frerichs RR: Accuracy of saliva test for HIV antibody. Journal of Acquired Immune Deficiency Syndrome and Human Retrovirology (1995) 10:484–485
Cribier B, Rey D, Schmitt C, Lang J-M, Kirn A, Stoll-Keller F: High hepatitis C viraemia and impaired antibody response in patients coinfected with HIV. AIDS (1995) 9:1131–1136
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Bello, P.Y., Pasquier, C., Gourney, P. et al. Assessment of a hepatitis C virus antibody assay in saliva for epidemiological studies. Eur. J. Clin. Microbiol. Infect. Dis. 17, 570–572 (1998). https://doi.org/10.1007/BF01708621
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DOI: https://doi.org/10.1007/BF01708621