Abstract
RNA from potato virus S (PVS), a member of the carlavirus group, was translated in vitro in rabbit reticulocyte lysate. Time-course experiments revealed the largest product of Mr 190 kD, decreasing in intensity after 60-min incubations, correlating with the accumulation of a 150-kD peptide. This apparent processing could be blocked by the addition of the amino-acid analogues p-fluorophenylalanine and L-canavanine for phenylalanine and arginine, respectively. L-canavanine also appeared to specifically reduce the quantity of PVS (34 kD) coat protein, concomitant with the synthesis of a 36-kD peptide. Sucrose gradient-fractionated genomic RNA directed the synthesis of predominantly 190-kD peptides that appeared not to be processed in the absence of small molecular weight (subgenomic) RNA products.
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References
Matthews, R.E.F., Intervirology12 131–296, 1979.
Mackenzie, D.J., Tremaine, J.H., and Stace-Smith, R., J Gen Virol70 1053–1063, 1989.
Rupasov, V.V., Morozov, S., Yu, Kanyuka, K.V., and Zavrier, S.K., J Gen Virol70 1861–1869, 1989.
Memelink, J., Van der Vlugt, I.M.C., Linthorst, H.J.M., Derks, A.F.L.M., Aojes, C.J., and Bol, J.F., J Gen Virol71 917–924, 1990.
Meehan, B.M. and Mills, P.R., Intervirology32 262–267, 1991.
Foster, G.D., Millar, A.W., Meehan, B.M., and Mills, P.R., J Gen Virol71 1877–1880, 1990.
Foster, G.D. and Mills, P.R., Virus Res17 61–70, 1990.
Foster, G.D. and Mills, P.R., J Gen Virol71 1247–1249, 1990.
Foster, G.D. and Mills, P.R., Acta Virol35 260–267, 1991.
Foster, G.D. and Mills, P.R., Virus Genes5 327–336, 1991.
Meehan, B.M. and Mills, P.R., Virus Genes5 175–181, 1991.
Foster, G.D. and Mills, P.R., Potato Res33 487–495, 1990.
Shields, S.A. and Wilson, T.M.A., J Gen Virol68 169–180, 1987.
Harbison, S.A., Wilson, M.A., and Davies, J.W., Biosci Rep 4, 949–956, 1984.
Laemmli, U.K., Nature (London)227 680–685, 1970.
Pelham, H.R.B., Virology96 463–477, 1979.
Vance, V.B. and Beachy, R.N., Virology132 271–281, 1984.
Mumford, R.A., Pickett, C.B., Zimmerman, M., and Strauss, A.W., Biochem Biophys Res Commun103 565–572, 1981.
Strauss, J.H., Strauss, E.G., Hahn, C.S., Hahn, Y.S., Galler, R., Hardy, W.R., and Rice, C.M.,Proceedings of the First International Symposium on Positive Strand RNA Viruses. 1987, pp. 209–225.
Boege, U., Wentlger, G., Wengler, G., and Wittman-Liebold, B., Virology113 293–303, 1981.
Krausslich, H.G., and Wimmer, E., Ann Rev Biochem57 701–754, 1988.
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Foster, G.D., Mills, P.R. Translation of potato virus S RNA in vitro: Evidence of protein processing. Virus Genes 6, 47–52 (1992). https://doi.org/10.1007/BF01703756
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DOI: https://doi.org/10.1007/BF01703756