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Immunological characterization of canine hematopoietic progenitor cells

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Summary

Canine hematopoietic progenitor cells were characterized by separation with monoclonal antibodies. Depleted and enriched fractions were studied for growth of CFU-GM in semisolid agar and for repopulating capacity of lethally irradiated dogs. CFU growth was not reduced by depletion of marrow using monoclonal antibodies F 3-20-7 (anti-dog Thy-1), MT606 (anti-human CD6), and IOT2a (anti-human DR). CFU growth was variable following treatment with the anti-canine T-cell antibody MdT-P1 and immunomagnetic bead separation. It was regularly enriched when MdT-P1 treatment was followed by immunorosetting with staphylococcal protein A-loaded sheep red blood cells and density gradient separation. Lethally irradiated dogs were reconstituted by autologous marrow depleted of MdT-P1-positive cells using immunorosetting and density gradient centrifugation, whereas immunomagnetic bead-depleted marrow was ineffective. Fluorescence-activated cell sorting showed enrichment of hematopoietic progenitor cells in the weakly MdT-P1-positive fraction.

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Authors and Affiliations

  1. Institut für Immunologie, Hämatologikum der GSF, Marchioninistrasse 25, W-8000, München 70, FRG

    J. Hahn, H. J. Kolb, M. Schumm, J. Maldacker, N. Schwella & S. Thierfelder

  2. Experimentelle Hämatologie, Hämatologikum der GSF, Marchioninistrasse 25, W-8000, München 70, FRG

    J. Ellwart

  3. Klinische Hämatologie, Hämatologikum der GSF, Marchioninistrasse 25, W-8000, München 70, FRG

    K. Beisser, L. K. Lösslein, E. Holler & W. Wilmanns

  4. Medizinische Klinik III, Klinikum Großhadern, Marchioninistrasse 15, W-8000, München 70, FRG

    H. J. Kolb, E. Holler & W. Wilmanns

  5. Institut für Immunologie, Ludwig-Maximilian-Universität München, Goethestrasse 31, W-8000, München 2, FRG

    P. Rieber

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  1. J. Hahn
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  2. H. J. Kolb
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  3. M. Schumm
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  4. K. Beisser
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  7. J. Maldacker
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  8. N. Schwella
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  9. L. K. Lösslein
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  10. E. Holler
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Additional information

Nonreviewed contribution to the annual meeting of the German and Austrian Societies of Hematology.

This work was supported by the Wilhelm Sander Foundation, AZ 84.020.1

This work is part of a doctoral thesis by J. Hahn, Ludwig-Maximilian-University Munich

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Hahn, J., Kolb, H.J., Schumm, M. et al. Immunological characterization of canine hematopoietic progenitor cells. Ann Hematol 63, 223–226 (1991). https://doi.org/10.1007/BF01703448

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  • DOI: https://doi.org/10.1007/BF01703448

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