Summary
Several flow-cytometric methods for reticulocyte enumeration in whole blood have been developed, with different degrees of practical use. Recently, a new fluorochrome, 1′,3′-diethyl-4,2′-quinolylthyacianine (DEQTC) was proposed in a brief report, as an alternative to thiazole orange for reticulocyte counting. We have evaluated the usefulness of this fluorescent stain by assessing the optimal conditions for the flow-cytometric analysis, and by comparing in double-blind assays the quantitative results of this technique with those obtained by manual counting with brilliant cresyl blue. Our results show that flow cytometry with DEQTC is highly correlated to the manual method (r=0.95–0.99), supporting the interest of this particular stain and of flow cytometry for routine laboratory work in hematology.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bockstaele DR van, Peetermans ME (1989) 1′,3′-Diethyl-4,2′-quinolylthiacyanine iodide as a “thiazole orange” analogue for nucleic acid staining. Cytometry 10: 214–216
Corash L, Rheinschmidt M, Lieu S, Meers P, Brew E (1988) Enumeration of reticulocytes using fluorescence-activated flow cytometry. Pathol Immunopathol Res 7: 381–394
Corash L, Rheinschmidt M, Lieu S, Meers P, Brew E (1988) Fluorescence-activated flow cytometry in the hematology clinical laboratory. Cytometry [Suppl] 3: 60–64
Dacie JV, Lewis SM (1984) The reticulocyte count. In: Dacie JV, Lewis SM (eds) Practical haematology, 5th edn. Churchill Livingstone, London, pp 58–60
Davis BH, Bigelow NC (1989) Flow cytometric reticulocyte quantification using thiazole orange provides clinically useful reticulocyte maturity index. Arch Pathol Lab Med 113: 684–689
Davis BH, Bigelow NC (1990) Clinical flow cytometric reticulocyte analysis. Pathobiology 58: 99–106
Eder H, Fritsch H (1989) Automated microfluorometric absolute count and maturation analysis of reticulocytes. Klin Wochenschr 67: 1048–1057
Ferguson DJ, Lee SF, Gordon PA (1990) Evaluation of reticulocyte counts by flow cytometry in a routine laboratory. Am J Hematol 33: 13–17
Hackney JR, Cembrowski GS, Prystowsky MB, Kant JA (1989) Automated reticulocyte counting by image analysis and flow cytometry. Lab Med 20: 551–555
Koepke JF, Koepke JA (1986) Reticulocytes. Clin Lab Haematol 8: 169–179
Kojima K, Niri M, Setoguchi K, Tsuda I, Tatsumi N (1989) An automated optoelectronic reticulocyte counter. Am J Clin Pathol 92: 57–61
Lee LG, Chen CH, Chiu LA (1986) Thiazole orange: a new dye for reticulocyte analysis. Cytometry 7: 508–517
Metzger DK, Charache S (1987) Flow cytometric reticulocyte counting with thioflavin T in a clinical hematology laboratory. Arch Pathol Lab Med 111: 540–544
Nobes PR, Carter AB (1990) Reticulocyte counting using flow cytometry. J Clin Pathol 43: 675–678
Peebles DA, Hochberg A, Clark TD (1981) Analysis of manual reticulocyte counting. Am J Clin Pathol 76: 713–717
Savage RA, Skoog DP, Rabinovitch A (1985) Analytical inaccuracy and imprecision in reticulocyte counting: a preliminary report from the College of American Pathologist's Reticulocyte Project. Blood Cells 11: 97–112
Shapiro HM (1988) Practical flow cytometry, 2nd edn. Liss, New York
Tanke HJ, Nieuwenhuis IAB, Koper GJM, Slats JCM, Ploem JS (1980) Flow cytometry of human reticulocytes based on RNA fluorescence. Cytometry 1: 313–320
Tanke HJ, Rothbarth PH, Vossen JM, Koper GJM, Ploem JS (1983) Flow cytometry of reticulocytes applied to clinical hematology. Blood 61: 1091–1097
Valet G (1984) A new method for fast blood cell counting and partial differentiation by flow cytometry. Blut 49: 83–90
Vaugham WP, Hall J, Dougherty D, Peebles D (1985) Simultaneous reticulocyte and platelet counting on a clinical flow cytometer. Am J Hematol 18: 385–391
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Guasch, R., Juan, G., Carretero, F. et al. Flow-cytometric enumeration of reticulocytes with the new fluorochrome 1′,3′-diethyl-4,2′-quinolylthiacyanine. Ann Hematol 65, 184–187 (1992). https://doi.org/10.1007/BF01703112
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01703112