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Molecular genetic analysis of DNA obtained from fixed, air dried or paraffin embedded sources

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Summary

In the present study methods are described for the analysis of the genomic structure of DNA isolated from tissues that have been stored up to several years as air-dried or stained bone marrow smears, bone marrow biopsies in “Histicon”, chromosomal preparations in fixative or as formalin fixed tissues embedded in paraffin. By application of the new techniques clonal B- and T-cell disorders, Philadelphia chromosome translocations and ras oncogene mutations in pancreatic carcinomas could be detected. Thus, these DNA extraction procedures may open new avenues to pathological archives and enable the analysis of samples when fresh material is not available.

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Authors and Affiliations

  1. Department of Internal Medicine, University of Innsbruck, Anichstrasse 35, A-6020, Innsbruck, Austria

    K. Grünewald

  2. Department of Pediatrics II, Laboratory of Molecular Biology, Oberer Eselsberg 10, W-7900, Ulm, Federal Republic of Germany

    K. Grünewald, J. Lyons, T. E. Hansen-Hagge, J. W. G. Janssen & C. R. Bartram

  3. Department of Pathology, University of Innsbruck, Müllerstrasse 44, A-6020, Innsbruck, Austria

    H. Feichtinger

  4. CETUS Corporation, 1400 Fifty-Third Street, 94149, Emeryville, CA, USA

    J. Lyons

Authors
  1. K. Grünewald
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  2. J. Lyons
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  3. T. E. Hansen-Hagge
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  4. J. W. G. Janssen
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  5. H. Feichtinger
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  6. C. R. Bartram
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Additional information

This work is dedicated to Prof. Dr. Dr. Dres h. c. H. Braunsteiner on the occasion of his award of honorary doctorship by the New York Medical School, by the University of Munich, and by the University of Budapest.

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Grünewald, K., Lyons, J., Hansen-Hagge, T.E. et al. Molecular genetic analysis of DNA obtained from fixed, air dried or paraffin embedded sources. Ann Hematol 62, 108–114 (1991). https://doi.org/10.1007/BF01702923

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  • DOI: https://doi.org/10.1007/BF01702923

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