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Nucleotide sequence of a hop stunt viroid variant isolated from citrus growing in Taiwan

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Abstract

The 303 nucleotide sequence of HSVd-citrus(T), a hop stunt viroid (HSVd) variant present in Etrog citron growing in Taiwan, was determined from cDNAs amplified by the polymerase chain reaction. HSVd-citrus(T) is very similar to several HSVd isolates previously recovered from citrus or cucumber, and exhibits microsequence heterogeneity at positions 154 and 181. Phylogenetic analysis using maximum parsimony grouped HSVd-citrus(T) with seven other isolates from citrus and cucumber in a large cluster of “citrus-type” isolates. A similar analysis revealed marked differences in both the extent and distribution of sequence variation among naturally occurring isolates of potato spindle tuber viroid.

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Authors and Affiliations

  1. Agricultural Biotechnology Laboratory, National Chung-Hsing University, Taichung, Taiwan 402, Republic of China

    Yau-Heiu Hsu & Wei Chen

  2. Molecular Plant Pathology Laboratory, Plant Sciences Institute, USDA-ARS, Beltsville, Maryland, USA

    Robert A. Owens

Authors
  1. Yau-Heiu Hsu
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  2. Wei Chen
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  3. Robert A. Owens
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Hsu, YH., Chen, W. & Owens, R.A. Nucleotide sequence of a hop stunt viroid variant isolated from citrus growing in Taiwan. Virus Genes 9, 193–195 (1995). https://doi.org/10.1007/BF01702664

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  • DOI: https://doi.org/10.1007/BF01702664

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