Abstract
Two or three regions containing three or more successive newly defined heptads of a–d hydrophobic amino acid repeats have been located in the cDNA-derived amino acid sequences of glycoprotein G of all rhabdoviruses examined (rabies, vesicular stomatitis, fish, and plant rhabdoviruses) by computer search. These new heptad-repeats differ from those previously reported in other viruses because of the presence of all the hydrophobic amino acids in positions a or d, and because they are not predicted to form coiled coils by current methods and thus they have not been detected previously in any rhabdoviruses. The two or three heptad-repeat regions were the only parts of the glycoprotein with at least three successive heptad-repeats in all the rhabdoviral sequences studied and had low sequence variability among the members of each of the rhabdoviral genus but show no sequence similarity among the different genus. All these newly detected heptad repeats were in the vicinity of some of the higher hydrophobic regions in each of the rhabdovirus genera studied and were found mostly, but not always, outside the extra amino acid sequences that occur in the longer insect or plant rhabdovirus glycoprotein G. The correspondence of position and structure of these heptad-repeats among all the rhabdoviruses suggests its participation in common function(s), most probably related to viral fusion with cellular membranes.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Lupas A., Van Dyke M., and Stock J., Science252 1162–1169, 1991.
Chambers P., Pringle C.R., and Easton A.J., J Gen Virol71 3075–3080, 1990.
Bullough P.A., Hughson F.M., Skehel J.J., and Wiley D.C., Nature371 37–43, 1994.
Carr C.M. and Kim P.S., Cell73 823–832, 1993.
Zhang L. and Ghosh H.P., J Virol68 2186–2193, 1994.
Coll J.M., 1995, submitted.
Gaudin Y., Ruigrok R.W.H., Knossow M., and Flamand A.J., Virology67 1365–1372, 1993.
Gaudin Y., Ruigrok R.W.H., Tuffereau C., Knossow M., and Flamand A., Virology187 627–632, 1992.
Wunner W.H., Reagan K.J., and Koprowski H.J., Virology50 691–697, 1984.
Benmansour H., Leblois H., Coulon P., Tuffereau C., Gaudin Y., Flamand A., and Lafay F., J Virol65 4198–4203, 1991.
Lecocq-Xhonneux F., Thiry M., Dheur I., Rossius M., Vanderheijden N., Martial J., and DeKinkelin P., J Gen Virol75 1579–1587, 1994.
Bourhy H., Kissi B., and Tordo N., Virology194 70–81, 1993.
Nichol S.T., Rowe J.E., and Fitch W.M., Virology168 281–291, 1989.
Schulz G.E. and Schimer R.H. (eds.), Principles of Protein Structure, Springer-Verlag, New York, 1984, p. 2.
Bilsel P.A. and Nichol S.T., J Virol64 4873–4883, 1990.
Tordo N., Bourhy H., Sather S., and Ollo R., Virology194 59–69, 1993.
Li Y., Drone C., Sat E., and Ghosh H.P., J Virol67 4070–4077, 1993.
Lafon M., Wiktor T.J., and Macfarlan R.I., J Gen Virol64 843–851, 1983.
Seif G., Coulon P., Rollin P.E., and Flamand A.J., Virology53 926–934, 1985.
Wunner W.H., Dietzchold B., Smith C.L., and Lafon M., Virology140 1–12, 1985.
Estepa A., Thiry M., and Coll J.M., J Gen Virol75 1329–1338, 1994.
Thiry M., Lecoq-Xhonneux F., Dheur I., Renard A., and Kinkelin D., Vet. Microbiol23 221–226, 1991.
Lorenzen N., Olesen N.J., Vestergaard-Jorgensen P.E., Etzerodt M., Holtet T.L., and Thorgersen M.C., J Gen Virol74 623–630, 1993.
Thiry M., Lecoq-Xhonneux F., Dheur I., Renard A., and Kinkelin D., Biochim Biophys Acta1090 345–347, 1991.
Koener J.F., Passavant C.W., Kurath G., and Leong J., J Virol61 1342–1349, 1987.
Tordo N., Poch O., Ermine A., Keith G., and Rougeon F., Proc Natl Acad Sci USA83 3914–3918, 1986.
Anilionis A., Wunner W.H., and Curtis P.J., Nature294 275–278, 1981.
Conzelmann K.K., Cox J.H., Schneider L.G., and Thiel H.J., Virology175 485–489, 1990.
Morimoto K., Ohkubo A., and Kawai A., Virology173 465–477, 1989.
Tordo N., Mem Inst Butantan53 31–51, 1991.
Prehaud C., Takehara K., Flamand A., and Bishop D.H.L., Virology173 390–399, 1989.
Gallione C.J. and Rose J.K., J Virol54 374–382, 1985.
Rose J.K. and Gallione C.J., J Virol39 519–528, 1981.
Vandepol S.B., Le Francois L., and Holland J.J., Virology148 312–325, 1986.
Teninges D. and Bras-Herreng F., J Gen Virol68 2625–2638, 1987.
Walker P.J., Byrne K.A., Riding G.A., Cowley J.A., Wang Y., and McWilliam S., Virology191 49–61, 1992.
Goldberg B.K., Modrell B., Hillman B.I., Heaton A.L., Choi T., and Jackson O.A., Virology185 32–38, 1991.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Morales Coll, J. Heptad-repeat sequences in the glycoprotein of rhabdoviruses. Virus Genes 10, 107–114 (1995). https://doi.org/10.1007/BF01702591
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01702591