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Neutralizing monoclonal antibodies against α and β subunits of theUstilago maydis virus encoded toxin

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Abstract

The toxins secreted byUstilago maydis are encoded by dsRNA viruses. The KP6 toxin encoded by subtype P6 consists of two polypeptides α and β, which are not covalently bound. Neutralizing monoclonal antibodies (MoAbs) were raised against each subunit. Some of the anti-β MoAbs identify different epitopes in the antigen. The MoAbs were used to affinity purify α and β polypeptides from culture media and to detect the precursor of the mature toxin.

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Authors and Affiliations

  1. Department of Microbiology-Biotechnology, G.S. Wise Faculty of Life Sciences, Tel-Aviv University, 69978, Ramat-Aviv, Israel

    I. Ginzberg, S. Rosenblum & Y. Koltin

  2. The Hybridoma Unit, G.S. Wise Faculty of Life Sciences, Tel-Aviv University, Ramat-Aviv, Israel

    N. I. Smorodinsky

Authors
  1. I. Ginzberg
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  2. S. Rosenblum
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  3. Y. Koltin
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  4. N. I. Smorodinsky
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Ginzberg, I., Rosenblum, S., Koltin, Y. et al. Neutralizing monoclonal antibodies against α and β subunits of theUstilago maydis virus encoded toxin. Virus Genes 6, 273–280 (1992). https://doi.org/10.1007/BF01702565

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  • DOI: https://doi.org/10.1007/BF01702565

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