Abstract
To investigate the accuracy of the polymerase chain reaction (PCR) method for the detection ofToxoplasma gondii in clinical specimens, aliquots of amniotic fluid to which known amounts ofToxoplasma gondii DNA had been added were tested by five European Centres. Four laboratories were able to detect DNA at levels equivalent to ten tachyzoites or less, including two that detected DNA equivalent to a single parasite. Two laboratories erroneously found one of eight negative control samples to be positive. These findings confirm that the high level of sensitivity associated with the PCR method can be readily achieved under routine laboratory conditions, but they also underscore the potential for both false-positive and false-negative findings to occur. Furthermore, the results confirm the urgent need for an external quality assurance scheme to support laboratories employing PCR in a clinical context for the detection ofToxoplasma gondii.
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References
Grover CM, Thulliez P, Remington JS, Boothroyd JC: Rapid prenatal diagnosis of congenital toxoplasma infection by using polymerase chain reaction and amniotic fluid. Journal of Clinical Microbiology 1990, 28: 2297–2301.
Cazenave J, Forestier F, Bessieres MH, Broussin B, Begueret J: Contribution of a new PCR assay to the prenatal diagnosis of congenital toxoplasmosis. Prenatal Diagnosis 1992, 12: 119–127.
Dupouy-Camet J, Lavareda de Souza S, Maslo C, Paugam A, Saimot A, Benarous R, Tourte-Schaefer C, Derouin F: Detection ofToxoplasma gondii in venous blood from AIDS patients by polymerase chain reaction. Journal of Clinical Microbiology 1993, 31: 1866–1869.
Parmley SF, Goebel FD, Remington JS: Detection ofToxoplasma gondii in cerebrospinal fluid from AIDS patients by polymerase chain reaction. Journal of Clinical Microbiology 1992, 30: 3000–3002.
Filice GA, Hitt JA, Mitchell CD, Blackstad M, Sorensen SW: Diagnosis of toxoplasma parasitaemia in patients with AIDS by gene detection after amplification with polymerase chain reaction. Journal of Clinical Microbiology 1993, 31: 2327–2331.
Christina N, Pelloux H, Goulhot C, Brion JP, Leclerq P, Ambroise-Thomas P: Detection ofToxoplasma gondii in AIDS patients by the polymerase chain reaction. Infection 1993, 21: 150–153.
Roth A, Roth B, Hoffken G, Steuber S, Khalifa Kl, Janitschke K: Application of the polymerase chain reaction in the diagnosis of pulmonary toxoplasmosis in immunocompromised patients. European Journal of Clinical Microbiology & Infectious Diseases 1992, 11: 1177–1181.
Hohlfeld P, Daffos F, Costa J-M, Thulliez P, Forestier F, Vidaud M: Prenatal diagnosis of congenital toxoplasmosis with a polymerase chain reaction test on amniotic fluid. New England Journal of Medicine 1994, 331: 695–699.
Pelloux H, Weiss J, Simon J, Muet F, Fricker-Hidalgo H, Goullier-Feuret A, Ambroise-Thomas P: A new set of primers for the detection ofToxoplasma gondii in amniotic fluid using the polymerase chain reaction. FEMS Microbiology Letters 1996, 138: 11–15.
Guy EC, Joynson DHM: Potential of the polymerase chain reaction in the diagnosis of active toxoplasma infection by detection of parasite in blood. Journal of Infectious Diseases 1995, 172: 319–322.
Zaaijer HL, Cuypers HT, Reesink HW, Winkel IN, Gerken G, Lelie PN: Reliability of polymerase chain reaction for the detection of hepatitis C virus. Lancet 1993, 341: 722–724.
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Guy, E.C., Pelloux, H., Lappalainen, M. et al. Interlaboratory comparison of polymerase chain reaction for the detection ofToxoplasma gondii DNA added to samples of amniotic fluid. Eur. J. Clin. Microbiol. Infect. Dis. 15, 836–839 (1996). https://doi.org/10.1007/BF01701532
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DOI: https://doi.org/10.1007/BF01701532