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Molecular epidemiology of an outbreak of Legionnaires' disease associated with a cooling tower in Genova-Sestri Ponente, Italy

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Abstract

Fatty acid profile analysis, monoclonal antibody (MAb) subtyping, pulsed-field gel electrophoresis (PFGE), arbitrarily primed polymerase chain reaction (AP-PCR), and ribotyping were used to compare clinical and environmentalLegionella pneumophila serogroup 1 isolates from an outbreak of Legionnaires' disease presumptively associated with cooling towers. According to the Oxford subtyping scheme, the MAb subtype of patients' isolates and of two strains originating from a cooling tower was Pontiac, whereas the other isolates were subtype Olda. The strains showed no intrinsic strain-to-strain difference in fatty acid profiles, and ribotyping and length polymorphism of the 16S-23S rDNA intervening regions failed to reveal any differences between the isolates. Conversely, PFGE and AP-PCR appeared to be more discriminatory, as the same genomic profile was found for the clinical and some environmental strains. Meteorologic and epidemiological data and the results of molecular analysis of theLegionella pneumophila serogroup 1 isolates support the hypothesis that the infection was transmitted from one of the cooling towers to the indoor environment of the same building, to homes in proximity that had open windows, and to the streets. In fact, the outbreak diminished and later ended after a part in the tower was replaced. This investigation demonstrates the utility of combined molecular methods (i.e., phenotypic and genomic typing) in comparing epidemiologically linked clinical and environmental isolates. Finally, the outbreak confirms the risk of Legionnaires' disease posed by cooling towers, mainly when atmospheric thermal and humidity inversions occur. This finding emphasizes the need to determine whether the source of infection is in the living or working environment or somewhere else.

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Authors and Affiliations

  1. Laboratorio di Batteriologia e Micologia Medica, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161, Rome, Italy

    M. Castellani Pastoris, L. Ciceroni, S. Ciarrocchi, A. Pinto & P. Visca

  2. Istituto di Microbiologia, Université “La Sapienza”, Piazzale Aldo Moro 5, 00185, Rome, Italy

    P. Goldoni & L. Cattani

  3. Istituto di Igiene e Medicina Preventiva, Università di Genova, Via Pastore 1, 16132, Genoa, Italy

    R. Lo Monaco, B. Mentore & G. Flego

Authors
  1. M. Castellani Pastoris
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  2. L. Ciceroni
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  3. R. Lo Monaco
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  4. P. Goldoni
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  5. B. Mentore
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  6. G. Flego
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  7. L. Cattani
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  8. S. Ciarrocchi
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  9. A. Pinto
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  10. P. Visca
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Additional information

This work was partially supported by a grant from MURST (Ministero della Università e della Ricerca Scientifica e Tecnologica).

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Castellani Pastoris, M., Ciceroni, L., Lo Monaco, R. et al. Molecular epidemiology of an outbreak of Legionnaires' disease associated with a cooling tower in Genova-Sestri Ponente, Italy. Eur. J. Clin. Microbiol. Infect. Dis. 16, 883–892 (1997). https://doi.org/10.1007/BF01700554

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