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In vitro extracellular and intracellular activity of two newer and two earlier fluoroquinolones againstListeria monocytogenes

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Abstract

Two new fluoroquinolones (trovafloxacin and sparfloxacin) with enhanced activity against gram-positive pathogens and two earlier compounds (ciprofloxacin and ofloxacin) were tested for their in vitro inhibitory and bactericidal activity against 80 strains ofListeria monocytogenes. All strains were uniformly highly susceptible to trovafloxacin, the MIC90 being 0.25 mg/l. Resistance to sparfloxacin was not detected, however the MIC90 of sparfloxacin was eight times that of trovafloxacin. A few strains were resistant to ciprofloxacin and ofloxacin (MIC90 4 mg/l for both drugs). MBCs usually exceeded MICs by 2 to 4 times. The MBC90 of trovafloxacin (1 mg/l) was lower than that of the other three drugs (8 mg/l). After checking their ability to enter and grow within human enterocyte-like Caco-2 cells, four strains were used to study the intracellular activity and eradicating power of the four quinolones. Trovafloxacin was more active than sparfloxacin and the earlier fluoroquinolones in terms of both intracellular killing and inhibition of a cytopathogenic effect. The uniform high-level activity of trovafloxacin againstListeria monocytogenes isolates in conventional in vitro assays and its extracellular and intracellular killing of invasive strains suggest that this and maybe other new fluoroquinolones should be further investigated as possible anti-listerial agents.

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References

  1. Schuchat A, Swaminathan B, Broome CV: Epidemiology of human listeriosis. Clinical Microbiology Reviews 1991, 4: 169–183.

    PubMed  Google Scholar 

  2. Lorber B: Listeriosis. Clinical Infectious Diseases 1997, 24: 1–9.

    PubMed  Google Scholar 

  3. Farber JM, Peterkin PI:Listeria monocytogenes, a foodborne pathogen. Microbiological Reviews 1991, 55: 476–511.

    PubMed  Google Scholar 

  4. Schuchat A, Deaver KA, Wenger JD, Plikaytis BD, Mascola L, Pinner RW, Reingold AL, Broome CV, and the Listeria Study Group: Role of foods in sporadic listeriosis. I: Case-control study of dietary risk factors. Journal of the American Medical Association 1992, 267: 2041–2045.

    PubMed  Google Scholar 

  5. Pinner RW, Schuchat A, Swaminathan B, Hayes PS, Deaver KA, Weaver RE, Plikaytis BD, Reeves M, Broome CV, Wenger JD, and the Listeria Study Group: Role of foods in sporadic listeriosis. II: Microbiologie and epidemiologic investigation. Journal of the American Medical Association 1992, 267: 2046–2050.

    PubMed  Google Scholar 

  6. Jones EM, MacGowan AP: Antimicrobial chemotherapy of human infection due toListeria monocytogenes. European Journal of Clinical Microbiology & Infectious Diseases 1995, 14: 165–175.

    Google Scholar 

  7. Hof H, Nichterlein T, Kretschmar M: Management of listeriosis. Clinical Microbiology Reviews 1997, 10: 345–357.

    PubMed  Google Scholar 

  8. Charpentier E, Gerbaud G, Jacquet C, Rocourt J, Courvalin P: Incidence of antibiotic resistance inListeria species. Journal of Infectious Diseases 1995, 172: 277–281.

    PubMed  Google Scholar 

  9. Marget W, Seeliger HPR:Listeria monocytogenes: therapeutic possibilities and problems. Infection 1988, 16, Supplement 2: 175–177.

    PubMed  Google Scholar 

  10. Southwick FS, Purich DL: Intracellular pathogenesis of listeriosis. New England Journal of Medicine 1996, 334: 770–776.

    PubMed  Google Scholar 

  11. van den Broek PJ: Antimicrobial drugs, microorganisms, and phagocytes. Reviews of Infectious Diseases 1989, 11: 213–245.

    PubMed  Google Scholar 

  12. Hof H: Intracellular microorganisms: a particular problem for therapy. Infection 1991, 19, Supplement 4: 193–194.

    Google Scholar 

  13. Piddock LJV: New quinolones and gram-positive bacteria. Antimicrobial Agents and Chemotherapy 1994, 38: 163–169.

    PubMed  Google Scholar 

  14. Eliopoulos GM: In vitro activity of fluoroquinolones against gram-positive bacteria. Drugs 1995, 49, Supplement 2: 48–57.

    PubMed  Google Scholar 

  15. Nakamura S, Minami A, Nakata K, Kurobe N, Kouno K, Sakaguchi Y, Kashimoto S, Yoshida H, Kojima T, Ohue T, Fujimoto K, Nakamura M, Hashimoto M, Shimizu M: In vitro and in vivo antibacterial activity of AT-4110, a new broad-spectrum quinolone. Antimicrobial Agents and Chemotherapy 1989, 33: 1167–1173.

    PubMed  Google Scholar 

  16. Kojima T, Inoue M, Mitsuhashi S: In vitro activity of AT-4110 against clinical bacterial isolates. Antimicrobial Agents and Chemotherapy 1989, 33: 1980–1988.

    PubMed  Google Scholar 

  17. Chin NX, Gu JW, Yu KW, Zhang YX, Neu HC: In vitro activity of sparfloxacin. Antimicrobial Agents and Chemotherapy 1991, 35: 567–571.

    PubMed  Google Scholar 

  18. Briggs Gooding B, Jones RN: In vitro antimicrobial activity of CP-99,219, a novel azabicyclo-naphthyridone. Antimicrobial Agents and Chemotherapy 1993, 37: 2615–2622.

    Google Scholar 

  19. Eliopoulos GM, Klimm K, Eliopoulos CT, Ferraro MJ, Moellering RC: In vitro activity of CP-99,219, a new fluoroquinolone, against clinical isolates of gram-positive bacteria. Antimicrobial Agents and Chemotherapy 1993, 37: 366–370.

    PubMed  Google Scholar 

  20. Neu HC, Chin NX: In vitro activity of the fluoroquinolone CP-99,219. Antimicrobial Agents and Chemotherapy 1994, 38: 2615–2622.

    PubMed  Google Scholar 

  21. Pine L, Weaver RE, Carlone GM, Pienta PA, Rocourt J, Goebel W, Kathariou S, Bibb WF, Malcolm GB:Listeria monocytogenes ATCC 35152 and NCTC 7973 contain a nonhemolytic, nonvirulent variant. Journal of Clinical Microbiology 1987, 25: 2247–2251.

    PubMed  Google Scholar 

  22. Facinelli B, Varaldo PE, Toni M, Casolari C, Fabio U: Ignorance about listeria. British Medical Journal 1989, 299: 738.

    PubMed  Google Scholar 

  23. Facinelli B, Giovanetti E, Varaldo PE, Casolari C, Fabio U: Antibiotic resistance in foodborne listeria. Lancet 1991, 338: 1272.

    Google Scholar 

  24. Facinelli B, Giovanetti E, Casolari C, Varaldo PE: Interactions with lectins and agglutination profiles of clinical, food, and environmental isolates ofListeria. Journal of Clinical Microbiology 1994, 32: 2929–2935.

    PubMed  Google Scholar 

  25. Roberts MC, Facinelli B, Giovanetti E, Varaldo PE: Transferable erythromycin resistance inListeria spp. isolated from food. Applied and Environmental Microbiology 1996, 62: 269–270.

    PubMed  Google Scholar 

  26. Swaminathan B, Rocourt J, Bille J:Listeria. In: Murray PR, Baron EJ, Pfaller MA, Tenover FC, Yolken RH (ed): Manual of clinical microbiology. American Society for Microbiology, Washington, DC, 1995, p. 341–348.

    Google Scholar 

  27. National Committee for Clinical Laboratory Standards: Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. Approved standard M7-A4. NCCLS, Villanova, PA, 1997.

    Google Scholar 

  28. Comité de l'Antibiogramme de la Société Française de Microbiologie: Statement 1996 CA-SFM. Zone sizes and MIC breakpoints for non-fastidious organisms. Clinical Microbiology and Infection 1996, 2, Supplement 1: 46–49.

    Google Scholar 

  29. Cormican GC, Jones RN: In-vitro activity of trovafloxacin (CP 99,129) tested by two methods against 150 vancomycin-resistant enterococcal isolates. Journal of Antimicrobial Chemotherapy 1996, 37: 847–849.

    PubMed  Google Scholar 

  30. National Committee for Clinical Laboratory Standards: Methods for determining bactericidal activity of antimicrobial agents. Tentative guidelines M26-T. NCCLS, Villanova, PA, 1992.

    Google Scholar 

  31. Pascual A, Garcia I, Ballesta S, Perea EJ: Uptake and intracellular activity of trovafloxacin in human phagocytes and tissue-cultured epithelial cells. Antimicrobial Agents and Chemotherapy 1997, 41: 274–277.

    PubMed  Google Scholar 

  32. Appleman MD, Cherubin CE, Heseltine PNR, Stratton CW: Susceptibility testing ofListeria monocytogenes: a reassessment of bactericidal activity as a predictor for clinical outcome. Diagnostic Microbiology and Infectious Disease 1991, 14: 311–317.

    PubMed  Google Scholar 

  33. Nichterlein T, Kretschmar M, Budeanu C, Bauer J, Linss W, Hof H: Bay Y 318, a new quinolone derivative, rapidly eradicatesListeria monocytogenes from infected mice and L929 cells. Antimicrobial Agents and Chemotherapy 1994, 38: 1501–1506.

    PubMed  Google Scholar 

  34. Michelet C, Avril JL, Cartier F, Berche P: Inhibition of intracellular growth ofListeria monocytogenes by antibiotics. Antimicrobial Agents and Chemotherapy 1994, 38: 438–446.

    PubMed  Google Scholar 

  35. Michelet C, Avril JL, Arvieux C, Jacquelinet C, Cartier F: Comparative activities of new fluoroquinolones, alone and in combination with amoxicillin, trimethoprim-sulfamethoxazole, or rifampin, against intracellularListeria monocytogenes. Antimicrobial Agents and Chemotherapy 1997, 41: 60–65.

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Institute of Microbiology, University of Ancona Medical School, Via Ranieri, Monte d'Ago, 60131, Ancona, Italy

    B. Facinelli, G. Magi & P. E. Varaldo

  2. Department of Molecular, Cellular and Animal Biology, Via Camerini 4, 62032, Camerino, Italy

    M. Prenna & S. Ripa

Authors
  1. B. Facinelli
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  2. G. Magi
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  3. M. Prenna
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  4. S. Ripa
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  5. P. E. Varaldo
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Facinelli, B., Magi, G., Prenna, M. et al. In vitro extracellular and intracellular activity of two newer and two earlier fluoroquinolones againstListeria monocytogenes . Eur. J. Clin. Microbiol. Infect. Dis. 16, 827–833 (1997). https://doi.org/10.1007/BF01700413

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