Summary
Superoxide anion (O2 −) production and expression of cytochrome b558 by neutrophils were determined in 20 patients with myelodysplastic syndrome (MDS). The reduction of O2 − production was noted in eight of the 20 patients and an increase was noted in four patients when neutrophils were stimulated byn-formylmethionyl-leucyl-phenylalanine (FMLP), while a low level of O2 − production was found in 11 and an increase in six MDS patients when they were stimulated by phorbol myristate acetate (PMA). Among them, seven patients showed a decrease and four an increase in O2 − production on stimulation with either FMLP or PMA. Expression of cytochrome b 558 was found to be at low levels in patients who had neutrophils showing decreased O2 − production when stimulated with FMLP, indicating that decreased expression of cytochrome b558 might contribute to the impairment of O2 − production in some MDS patients. In this study, no significant differences in O2 − production were noted among subtypes of MDS; however, the patients who had received prednisolone showed lower levels of O2 − production than those who had not received prednisolone. Patients manifesting episodes of infection had reduced levels of O2 − production compared with those without infection. Furthermore, the fact that one patient who exhibited a marked reduction in neutrophil counts together with reduced O2 − production died of fatal infection, suggests that the determination of O2 − production, in combination with hematological features, may be of some help in predicting severe infection.
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Itoh, Y., Kuratsuji, T., Aizawa, S. et al. Superoxide anion production and expression of cytochrome b 558 by neutrophils are impaired in some patients with myelodysplastic syndrome. Ann Hematol 63, 270–275 (1991). https://doi.org/10.1007/BF01698377
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DOI: https://doi.org/10.1007/BF01698377