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Hematopoietic reconstitution after high-dose chemotherapy and autologous nonfrozen bone marrow rescue

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Summary

The kinetics of marrow engraftment were analyzed in 50 patient with acute leukemia (21), malignant lymphoma (15), and solid tumors (14) after high-dose multiagent chemotherapy followed by autologous bone marrow transplantation (ABMT) with nonfrozen bone marrow. Unseparated heparinized whole bone marrow was stored in 10% CPDA1 at 4°C for 72 h, then filtered and reinfused. The median number of nucleated cells reinfused was 1.6×108/kg (range 0.5–3.8×108/kg). All patients had a full hematopoietic reconstitution. Median time to achieve a neutrophil count > 500/μl was 20 days (range 12–39) and median time to achieve an unsupported platelet count > 20.000/μl was 20 days (range 10–55). The main factor associated with delayed engraftment was the number of prior chemotherapy cycles. We conclude that high-dose chemotherapy with nonfrozen ABMT is a safe procedure, without the requirement for costly cryopreservation facilities.

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Authors and Affiliations

  1. Zentrum für Innere Medizin, Abt. Hämatologie/Onkologie, Philipps-Universität, Baidinger Str., 3550, Marburg, FRG

    H. Köppler, K. H. Pflüger & K. Havemann

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  1. H. Köppler
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  2. K. H. Pflüger
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  3. K. Havemann
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Köppler, H., Pflüger, K.H. & Havemann, K. Hematopoietic reconstitution after high-dose chemotherapy and autologous nonfrozen bone marrow rescue. Ann Hematol 63, 253–258 (1991). https://doi.org/10.1007/BF01698374

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  • DOI: https://doi.org/10.1007/BF01698374

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