Summary
Mean lactic dehydrogenase (LDH) activity of human platelets is about 5 nU/platelet; expressed on a cell volume basis, it is 0.5 nU/fl. Platelet separation by means of centrifugation on a Percoll discontinuous gradient gives different subpopulations with a clear-cut change of LDH at a mean platelet volume (MPV) of about 6 fl. The LDH content of platelets with a MPV >6 fl is constant (about 0.5 nU/fl), while that of small platelets (3<MPV<6 fl) is inversely correlated with the MPV and reaches a value of 1.5–2.0 nU/fl. Because small platelets are granule-depleted, we suggest that platelet LDH activity be considered as an in vivo activation index.
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De Sole, P., Zappacosta, B. Platelet heterogeneity based on lactic dehydrogenase activity. Ann Hematol 65, 135–137 (1992). https://doi.org/10.1007/BF01695813
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DOI: https://doi.org/10.1007/BF01695813