Skip to main content
SpringerLink
Log in

Detection of vertical transmission of human immunodeficiency virus type 1 by a commercial polymerase chain reaction assay

  • Notes
  • Published:
European Journal of Clinical Microbiology and Infectious Diseases Aims and scope Submit manuscript

Abstract

In a prospective study, a commercial polymerase chain reaction (PCR) system was compared with a conventional procedure, based on PCR and hybridization with a radio-labeled probe, for the detection of human immunodeficiency virus (HIV) infection in 131 blood samples from 80 children born to HIV-seropositive mothers. Twenty-three of these children were HIV infected. The sensitivity and specificity of the commercial assay as compared with the conventional PCR procedure were 100% and 95.1%, respectively. This commercial method simplifies the performance of the conventional PCR technique and can be used to detect HIV type 1 vertical transmission.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. The European Collaborative Study: Risk factors for mother to child transmission of HIV-1. Lancet 1992, 339: 1007–1012.

    Google Scholar 

  2. Levy JA: HIV and the pathogenesis of AIDS. American Society for Microbiology, Washington, DC, 1994, p. 22–26.

    Google Scholar 

  3. De Rossi A, Ades AE, Mammano F, del Mistro A, Amadori A, Giaquinto C, Chieco-Bianchi L: Antigen detection, virus culture, polymerase chain reaction, and in vitro antibody production in the diagnosis of vertically transmitted HIV-1 infection. AIDS 1991, 5: 15–20.

    PubMed  Google Scholar 

  4. Quinn TC, Kline R, Moss MW, Livingston RA, Hutton N: Acid dissociation of immuno complexes improves diagnostic utility of p24 antigen detection in perinatally acquired human immunodeficiency virus infection. Journal of Infectious Diseases 1993, 167: 1193–1196.

    PubMed  Google Scholar 

  5. Brandt CD, Rakusan TA, Sison AV, Josephs SH, Saxena ES, Herzog KD, Parrott RH, Sever JL: Detection of human immunodeficiency virus type 1 infection in young pediatric patients by using polymerase chain reaction and biotinylated probes. Journal of Clinical Microbiology 1992, 30:36–40.

    PubMed  Google Scholar 

  6. Hammer S, Crumpacker C, D'Aquila R, Jackson B, Lathey J, Livnat D, Reichelderfer P: Use of virologic assays for detection of human immunodeficiency virus in clinical trials: recommendation of the AIDS Clinical Trials Group Virology Committee. Journal of Clinical Microbiology 1993, 31: 2557–2564.

    PubMed  Google Scholar 

  7. Jackson JB, Drew J, Lin HJ, Otto P, Bremer JW, Hollinger FB, Wolinsky SM, ACTG PCR Working Group, ACTG PCR Virology Laboratories: Establishment of a quality assurance program for human immunodeficiency virus type 1 DNA polymerase chain reaction assays by the AIDS clinical trials group. Journal of Clinical Microbiology 1993, 31: 3123–3128.

    PubMed  Google Scholar 

  8. Whetsall AJ, Drew JB, Milman G, Hoff R, Dragon EA, Adler K, Hui J, Otto P, Gupta P, Farzadegan H, Wolinsky SM: Comparison of three non radioisotopic polymerase chain reaction based methods for detection of human immunodeficiency virus type 1. Journal of Clinical Microbiology 1992, 30: 845–853.

    PubMed  Google Scholar 

  9. Jackson JB, Ndugwa C, Mmiro F, Kataaha P, Guay L, Dragon EA, Goldfarb J, Olness K: Non-isotopic polymerase chain reaction methods for the detection of HIV-1 in Ugandan mothers and infants. AIDS 1991, 5: 1463–1467.

    PubMed  Google Scholar 

  10. Cassol S, Butcher A, Kinard S, Spadoro J, Sy T, Lapointe N, Read S, Gomez P, Fauvel M, Major C, O'Shaugnessy M: Rapid screening for early detection of mother to child transmission of human immunodeficiency virus type-1. Journal of Clinical Microbiology 1994, 32: 2641–2645.

    PubMed  Google Scholar 

  11. Kellog DE, Kwok S: Detection of human immunodeficiency virus. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (ed): PCR protocols: a guide to methods and applications. Academic, San Diego, 1990, p. 337–347.

    Google Scholar 

  12. Folks TM, Powel D, Lightfoote M, Koening S, Fauci AS, Benn S, Rabson D, Daugherty D, Gendelman HE, Hoggan MD, Venkatesan S, Martin MA: Biological and biochemical characterization of a cloned Leu 3− cell surviving infection with the AIDS retrovirus. Journal of Experimental Medicine 1986, 164: 280–290.

    PubMed  Google Scholar 

  13. Report of a Consensus Workshop, Siena, Italy, January 17–18, 1992: Early diagnosis of HIV infection in infants. Journal of the Acquired Immune Deficiency Syndrome 1992, 5: 1169–1178.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Servicio de Microbiología, Hospital 12 de Octubre, Cta. Andalucfa km 5.4, 28041, Madrid, Spain

    P. Bermúdez, P. Moreno, R. Delgado & A. R. Noriega

  2. Unidad de Immunodeficiencias Pediátricas, Hospital 12 de Octubre, Cta. Andalucfa km 5.4, 28041, Madrid, Spain

    J. T. Ramos & J. Ruiz-Contreras

Authors
  1. P. Bermúdez
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. P. Moreno
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. R. Delgado
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. J. T. Ramos
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. J. Ruiz-Contreras
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. A. R. Noriega
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bermúdez, P., Moreno, P., Delgado, R. et al. Detection of vertical transmission of human immunodeficiency virus type 1 by a commercial polymerase chain reaction assay. Eur. J. Clin. Microbiol. Infect. Dis. 15, 327–330 (1996). https://doi.org/10.1007/BF01695666

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01695666

Keywords

Search

Navigation