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A narrow range, medium molecular weight pentastarch reduces structural organ damage in a hyperdynamic porcine model of sepsis

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Abstract

Objective: to compare diafiltered 6% pentastarch (Pentafraction-PDP, MWn 120000 and MWw 280000) and native pentastarch (Pentaspan-PSP, MWn 63000 and MWw 264000 dalton) in a porcine model of faecal peritonitis.Design: Randomised prospective study in 12 adolescent pigs.Interventions: Prior to infection the study solution was infused to increase Qt by 25%. Thereafter adjustments in infusion rate were made (up to 1 l/h) in an attempt to maintain Qt at 25% above baseline values.Measurements and results: Animals were sacrificed at 8h. Tissue was excised from the right lobe of liver and from the right lung and fixed for later electron microscopy and digital morphometric analysis. Patent sinusoidal lumen was significantly greater in group PDP compared to PSP (11.3%±2.3% of liver tissue versus 4.8%±1.1%,p<0.05) and this was accounted for by a significantly lower proportion of sinusoidal lumen occluded with white cells (2.1%±0.6% versus 6.6%±1.9%,p<0.05). Similarly, patent capillary represented a significantly higher proportion of lung tissue for group PDP versus PSP (26.2%±1.9% versus 18.5%±2.7%,p<0.05). The arithmetic mean alveolar capillary barrier thickness was significantly greater in group PSP than in group PDP (4.3±03 μm versus 2.5±03 μm,p<0.01).Conclusions: The molecular weight profile of Pentafraction was associated with less structural organ damage including less tissue oedema and less white cell occlusion.

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Authors and Affiliations

  1. Bloomsbury Department of Intensive Care, Middlesex Hospital, Mortimer Street, W1N 8AA, London, UK

    A. R. Webb MB, MRCP & M. G. Mythen

  2. Department of Medicine, Division of Physiological Medicine, London, UK

    R. F. Moss, D. Tighe, N. Al-Saady & E. D. Bennett

  3. Department of Radiology, St., Georges Hospital Medical School, Tooting, London, UK

    A. E. Joseph

Authors
  1. A. R. Webb MB, MRCP
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  2. R. F. Moss
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  3. D. Tighe
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  4. M. G. Mythen
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  5. N. Al-Saady
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  6. A. E. Joseph
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  7. E. D. Bennett
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Webb, A.R., Moss, R.F., Tighe, D. et al. A narrow range, medium molecular weight pentastarch reduces structural organ damage in a hyperdynamic porcine model of sepsis. Intensive Care Med 18, 348–355 (1992). https://doi.org/10.1007/BF01694363

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  • DOI: https://doi.org/10.1007/BF01694363

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