Summary
A single tetrad in which one genetical marker had segregated irregularly was analyzed genetically by outcrossing each culture derived from the tetrad to other haploid clones. Regular segregation in the resultant hybrids indicated that the cultures were all haploid. The original ascus was tetratype proving that all four nuclei had survived after reduction. All clones were haploid proving that the irregularities could not have arisen from fusion following an extra mitosis. It is inferred that the extra recessive was the result of an interaction in the hybrid in which a dominant was converted into a recessive allele. The converted clone was identified by the intermediate character of its physiological activity.
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This work has been supported by grants from the National Cancer Institute of the National Institutes of Health, Public Health Service, Contracts C-1179 and C-2140 and Anheuser-Busch, Inc.
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Lindegren, C.C., Lindegren, G., Drysdale, R.B. et al. Genetical analysis of the clones from a single tetrad of saccharomyces showing non-Mendelian segragation. Genetica 28, 1–24 (1957). https://doi.org/10.1007/BF01694305
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DOI: https://doi.org/10.1007/BF01694305