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Analysis of the immunological humoral response toMycobacterium tuberculosis glycolipid antigens (DAT, PGLTb1) for diagnosis of tuberculosis in HIV-seropositive and -seronegative patients

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Abstract

Using an enzyme immunoassay (EIA) test, the concentrations of IgG antibodies against 2,3 diacyl trehalose (DAT) and phenolic glycolipid Tb1 (PGLTb1) were measured in the sera of 153 patients with active tuberculosis, 50 of whom were coinfected with HIV, and in the sera of 152 healthy blood donors, 149 asymptomatic HIV-seropositive patients, 12 HIV-seronegative patients with conditions simulating tuberculosis, 23 HIV-seropositive patients with disseminated infection caused by mycobacteria other than tuberculosis and 24 HIV-seropositive patients with pulmonary disease from whom mycobacteria was not isolated in culture. A slightly lower percentage (74 %) of the HIV-seropositive than the HIV-seronegative (77 %) tuberculosis patients were positive for anti-DAT and antiPGLTb1 IgG antibodies, with a specificity ranging from 91 to 95 %. There was no significant difference between EIA sensitivity in smear-positive and smear-negative patients with pulmonary tuberculosis for all HIV immune statuses and sites of disease (pulmonary vs. extrapulmonary). In HIV-seropositive patients, however, sensitivity was always lower for disseminated tuberculosis than for localized tuberculosis. Combining data for both the smear test and the EIA maximized sensitivity. The main value of the EIA test could be to provide early complementary information by antibody detection in patients with tuberculosis, particularly those with a negative smear test.

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Authors and Affiliations

  1. Service de Microbiologie, Hôpital Saint-Louis, 1 avenue Claude Vellefaux, 75475, Paris Cedex 10, France

    N. Simonney & P. H. Lagrange

  2. Service Maladies Infectieuses, Hôpital Saint-Louis, 1 avenue Claude Vellefaux, 75475, Paris Cedex 10, France

    J. M. Molina

  3. Service de Pneumologie, Hôpital Saint-Louis, 1 avenue Claude Vellefaux, 75475, Paris Cedex 10, France

    M. Molimard

  4. Service d'Immunohématologie, Hôpital Saint-Louis, 1 avenue Claude Vellefaux, 75475, Paris Cedex 10, France

    E. Oksenhendler

  5. Service de Maladies Infectieuses, Hôpital Bichat ClaudeBernard, Paris, France

    C. Perronne

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Simonney, N., Molina, J.M., Molimard, M. et al. Analysis of the immunological humoral response toMycobacterium tuberculosis glycolipid antigens (DAT, PGLTb1) for diagnosis of tuberculosis in HIV-seropositive and -seronegative patients. Eur. J. Clin. Microbiol. Infect. Dis. 14, 883–891 (1995). https://doi.org/10.1007/BF01691495

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