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Emergence of resistance to beta-lactam agents inPseudomonas aeruginosa with group I beta-lactamases in Spain

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Abstract

The contribution of induction and stable derepression of chromosomal class I β-lactamases to β-lactam antibiotic resistance was studied in clinical isolates ofPseudomonas aeruginosa collected from patients treated with β-lactam antibiotics. Multiple isolates from the same patient were characterized by O-serotyping as a primary screen, combined with pyocin typing. Sonicated extracts of cells were assayed for chromosomal and plasmid-mediated β-lactamases by isoelectric focusing and cloxacillin inhibition studies. The specific β-lactamase activity, basal and induced, with cefoxitin was determined to differentiate strains with inducible or derepressed production of the enzyme. Beta-lactamase induction was performed in each strain against the β-lactam agents used in the therapy of each patient. The observations showed that induction against older penicillins such as penicillin, amoxicillin, and amoxicillin/clavulanate resulted in a moderate to strong increase in β-lactamase activity, whereas the results obtained with first-generation cephalosporins varied with the β-lactam agent tested. Third-generation cephalosporins were weak inducers of β-lactamases, and their use as therapy preceded the appearance of strains that produce chromosomal group I β-lactamases constitutively. These strains showed a remarkable reduction in sensitivity to ureidopenicillins, carboxipenicillins, third-generation cephalosporins, and monobactams, but not to carbapenems.

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Authors and Affiliations

  1. Department of Immunology, Microbiology, and Parasitology, School of Pharmacy, University of Basque Country, Apartado de Correos 450, Vitoria-Gasteiz, Spain

    K. Colom & R. Cisterna

  2. Department of Immunology, Microbiology, and Parasitology, School of Medicine, University of Basque Country, Apartado de Correos 699, Bilbao, Spain

    A. Fdz-Aranguiz, E. Suinaga & R. Cisterna

  3. Service of Microbiology, Basurto Hospital, Bilbao, Spain

    R. Cisterna

Authors
  1. K. Colom
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  2. A. Fdz-Aranguiz
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  3. E. Suinaga
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  4. R. Cisterna
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Colom, K., Fdz-Aranguiz, A., Suinaga, E. et al. Emergence of resistance to beta-lactam agents inPseudomonas aeruginosa with group I beta-lactamases in Spain. Eur. J. Clin. Microbiol. Infect. Dis. 14, 964–971 (1995). https://doi.org/10.1007/BF01691378

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