Abstract
Paired samples of saliva and serum from 286 highrisk subjects were tested for HIV using two commercial enzyme immunoassays following the same WHO testing strategy supplemented with a Western blot technique. Agreement between HIV testing in both sample types was 98.9 % (95 % confidence interval: 97.7–100). The sensitivity of an EIA (Wellcozyme GACELISA) alone for saliva was 100% (133/133) and the specificity 98.7% (151/153). These results support previous data showing that in some situations saliva might be an effective alternative to serum for HIV testing.
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Johnson AM, Parry JV, Best SJ, Smith AM, de Silva M, Mortimer PP: HIV surveillance by testing saliva. AIDS 1988, 2: 369–371.
Major CJ, Read SE, Coates RA, Francis A, McLaughlin BJ, Millson M, Shepherd F, Fanning M, Calzavara L, MacFadden D, Johnson JK, O'Shaughnessy MV: Comparison of saliva and blood for human immunodeficiency virus prevalence testing. Journal of Infectious Diseases 1991, 163: 699–702.
Frerichs R, Silarug N, Eskes N, Pagcharoenpol P, Rodklai A, Thangsupachai S, Wongba C: Saliva-based HIV-testing in Thailand. AIDS 1994, 8: 885–894.
WHO/Global Programme on AIDS: HIV antibody detection in oral fluids. Weekly Epidemiological Record 1993, 19: 133–134.
Tamashiro H, Constantine NT: Serological diagnosis of HIV infection using oral fluid samples. Bulletin of the World Health Organization 1994, 72: 135–143.
Tamashiro H, Maskill W, Emmanuel J, Fauquex A, Sato P, Heymann D: Reducing the cost of HIV antibody testing. Lancet 1993, 342: 87–90.
Soto-Ramirez LE, Hernández-Gómez L, Sifuentes-Osorino J, Barriga-Angulo G, Durate de Lima D, López-Portillo M, Ruiz-Palacios GM: Detection of specific antibodies in gingival crevicular transudate by enzymelinked immunosorbent assay for diagnosis of human immunodeficiency virus type 1 infection. Journal of Clinical Microbiology 1992, 30: 2780–2783.
Sato PA, Maskill WJ, Tamashiro H, Heymann DL: Strategies for laboratory HIV testing: an examination of alternative approaches not requiring Western blot. Bulletin of the World Health Organization 1994, 72: 129–134.
Connell JA, Parry JV, Mortimer PP, Duncan J: Novel assay for the detection of immunoglobulin G antihuman immunodeficiency virus in untreated saliva and urine. Journal of Medical Virology 1993, 41: 159–164.
Frerichs RR, Eskes N, Htoon MT: Validity of three assays for HIV-1 antibodies in saliva. Journal of Acquired Immune Deficiency Syndrome and Human Retrovirology 1994, 7: 522–524.
Unlinked anonymous monitoring of HIV prevalence in England and Wales: 1990–92. Communicable Disease Report 1993, 3: R1–R11.
Behets FM, Edidi B, Quinn TC, Atikala L, Bishagara K, Nzila N, Laga M, Piot P, Ryder RW, Brown CC: Detection of salivary HIV-1-specific IgG antibodies in highrisk populations in Zaire. Journal of Acquired Immunodeficiency Syndrome 1991, 4: 183–187.
Goldberg DJ, Tappin DM, Cameron S, Frischer M, Green ST, Follet EAC: Saliva and HIV testing. Lancet 1993, 341: 382.
Frerichs R, Htoon MT, Eskes N, Lwin S: Comparison of saliva and serum for HIV surveillance in developing countries. Lancet 1992, 340: 1496–1499.
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Mayans, M.V., Casabona, J., Rabella, N. et al. Testing of saliva and serum for HIV in high-risk populations. Eur. J. Clin. Microbiol. Infect. Dis. 14, 710–713 (1995). https://doi.org/10.1007/BF01690881
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DOI: https://doi.org/10.1007/BF01690881