Skip to main content
SpringerLink
Log in

Association between HIV and other DNA viruses in vitro

  • Articles
  • Published:
European Journal of Clinical Microbiology and Infectious Diseases Aims and scope Submit manuscript

Abstract

To investigate the association of human immunodeficiency virus (HIV) with various DNA viruses, including hepatitis B virus (HBV), cytomegalovirus (CMV) and Epstein-Barr virus, (EBV), simultaneous detection of HIV p24 antigen, HBV surface antigen and DNA, CMV-DNA and EBV-DNA expression was performed in phytohemagglutinin-stimulated peripheral blood mononuclear (PBMC) culture supernatants obtained from 54 individuals at risk for HIV infection. HIV expression in PBMC culture supernatants never occurred alone; expression of other viruses was always detected in the 24 samples expressing HIV antigen in vitro. Furthermore, in 16 patients expression of other viruses was detected without HIV expression, and in 14 patients none of the tested viruses were detected. These results indicate a strong association between the presence of HIV antibody and expression of DNA viruses in vitro (p=0.0001). The coexpression of these viruses could be related to the evolution of HIV infection and AIDS.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Macher AM, Reichert CM, Strauss SE, Longo DL, Parillo J, Lane HC, Fauci AS, Rook AH, Manischewitz JF, Quinnan GV: Death in the AIDS patient. Role of cytomegalovirus. New England Journal of Medicine 1983, 309: 1454.

    Google Scholar 

  2. Mintz L, Drew WL, Miner RC, Braff EH:Cytomegalovirus infections in homosexual men. An epidemiologic study. Annals of Internal Medicine 1983, 99: 326–329.

    PubMed  Google Scholar 

  3. Quinnan GV, Masur H, Rook AH, Armstrong G, Frederick WR, Epstein J, Manischewitz JF, Macher AM, Jackson L, Ames J, Smith HA, Parker M, Pearson GR, Parrillo J, Mitchell C, Strauss SE: Herpesvirus infections in the acquired immunodeficiency syndrome. Journal of the American Medical Association 1984, 252: 72–77.

    PubMed  Google Scholar 

  4. Spector SA, Hirata KK, Neuman TR: Identification of multiple cytomegalovirus strains in homosexual men with acquired immunodeficiency syndrome. Journal of Infectious Diseases 1984, 150: 953–956.

    PubMed  Google Scholar 

  5. Bartholomew C, Saxinger WC, Clarke JW, Gail M, Dudgeon A, Mahabir B, Hull-Drysdale B, Cleghon F, Gallo RC, Blattner WA: Transmission of HTLV-I and HIV among homosexual men in Trinidad. Journal of the American Medical Association 1987, 257: 2604–2608.

    PubMed  Google Scholar 

  6. Gradilone A, Zani M, Barillari G, Modesti M, Agliano A, Maiorano G, Ortona L, Frati L, Manzari V: HTLV-I and HIV infections in drug addicts in Italy. Lancet 1986, ii: 753–754.

    Google Scholar 

  7. Robert-Guroff M, Weiss SH, Giron JA, Jennings AM, Ginzburg HM, Margolis IB, Blattner WA, Gallo RC: Prevalence of antibodies to HTLV-I -II, and -III in intravenous drug abusers from an AIDS-endemic region. Journal of the American Medical Association 1986, 255: 3133–3137.

    PubMed  Google Scholar 

  8. Colebunders R, Mann JM, Francis H, Bila K, Izaley L, Ilwaja M, Kakonde N, Quinn T, Curran T, Piot A: Herpes zoster in African patients: a clinical predictor of human immunodeficiency virus infection. Journal of Infectious Diseases 1988, 157: 314–318.

    PubMed  Google Scholar 

  9. Holmberg SD, Stuart JA, Gerber AR, Byers RH, Lee FK, O'Malley PM, Nahmias AJ: Prior herpes simplex virus type 2 infection as a risk factor for HIV infection. Journal of the American Medical Association 1988, 259: 1048–1050.

    PubMed  Google Scholar 

  10. Spector SA, Merrill R, Wolf D, Dankner W: Detection of human cytomegalovirus in plasma of AIDS patients during acute visceral disease by DNA amplification. Journal of Clinical Microbiology 1992, 30: 2359–2365.

    PubMed  Google Scholar 

  11. Ostrove J, Leonard M, Weck K, Rabson A, Gendelman H: Activation of the human immunodeficiency virus by herpes simplex virus type 1. Journal of Virology 1987, 61: 3726–3732.

    PubMed  Google Scholar 

  12. Skolnik PR, Kosloff BR, Hirsch MS: Bidirectional interactions between human immunodeficiency virus type 1 and cytomegalovirus. Journal of Infectious Diseases 1988, 157: 508–514.

    PubMed  Google Scholar 

  13. Lardelli P, Steinberg SM, Campelo C, Fernandez de Aranguiz A, Sarria L, Gorriño MT, Cisterna R: Evidence of an in vitro association between human immunodeficiency virus antigen p24 and Epstein-Barr virus DNA. European Journal of Clinical Microbiology & Infectious Diseases 1992, 11: 157–161.

    Google Scholar 

  14. Rinaldo CR, Kingsley LA, Lyter DW, Rabin BS, Atchison RW, Bodner AJ, Weiss SH, Saxinger WC: Association of HTLV-III with Epstein-Barr virus: infection and abnormalities of T-lymphocytes in homosexual men. Journal of Infectious Diseases 1986, 154: 556–561.

    PubMed  Google Scholar 

  15. Sumaya CV, Boswell RN, Ench Y, Kisner DL, Hersh EM, Reuben JM, Mansell PWA: Enhanced serological and virological findings of Epstein-Barr virus in patients with AIDS and AIDS-related complex. Journal of Infectious Diseases 1986, 154: 864–870.

    PubMed  Google Scholar 

  16. Vlach J, Pitha PM: Herpes simplex virus type 1-mediated induction of human immunodeficiency virus type 1 provirus correlates with binding of nuclear proteins to the NF-kB enhancer and leader sequence. Journal of Virology 1992, 66: 3616–3623.

    PubMed  Google Scholar 

  17. Ghazal P, Young J, Giulietti E, DeMattei C, Garcia J, Gaynor R, Stenberg RM, Nelson JA: A discrete cis element in the human immunodeficiency virus long terminal repeat mediates synergistic trans activation by cytomegalovirus immediate-early proteins. Journal of Virology 1991, 65: 6735–6742.

    PubMed  Google Scholar 

  18. Hook EW, Cannon RO, Nahimas AJ, Lee FF, Campbell CH, Glasser D, Quinn TC: Herpes simplex virus infection as a risk factor for human immunodeficiency virus infection in heterosexuals. Journal of Infectious Diseases 1992, 165: 251–255.

    PubMed  Google Scholar 

  19. Albrecht MA, DeLuca NA, Byrn RA, Schaffer PA, Hammer SM: The herpes simplex virus immediate-early protein, ICP4, is required to potentiate replication of human immunodeficiency virus in CD4+ lymphocytes. Journal of Virology 1989, 63: 1861–1868.

    PubMed  Google Scholar 

  20. Gimble JM, Duh E, Ostrove JM, Gendelman HE, Max EE, Rabson AB: Activation of the human immunodeficiency virus long terminal repeat by herpes simplex virus type 1 is associated with induction of a nuclear factor that binds to the NF-kB/core enhancer sequence. Journal of Virology 1988, 62: 4104–4112.

    PubMed  Google Scholar 

  21. Lathey JL, Spector DH, Spector SA: Human cytomegalovirus-mediated enhancement of human immunodeficiency virus type-1 production in monocyte-derived macrophages. Virology 1993, 199: 98–104.

    Google Scholar 

  22. Gendelman HE, Phelps W, Feigenbaum L, Ostrove JM, Adachi A, Howley PM, Khoury G, Ginsberg HS: Transactivation of the human immunodeficiency virus long terminal repeat sequence by DNA viruses. Proceedings of the National Academy of Sciences of the USA 1986, 83: 9759–9763.

    PubMed  Google Scholar 

  23. Dahl KE, Burrage T, Jones F, Miller G: Persistent nonproductive infection of Epstein-Barr virus-transformed human B lymphocytes by human immunodeficiency virus type 1. Journal of Virology 1990, 64: 1771–1783.

    PubMed  Google Scholar 

  24. Henderson EE, Yang JY, Zhang RD, Bealer M: Altered HIV expression and EBV-induced transformation in coinfected PBLs and PBL subpopulations. Virology 1991, 182: 186–198.

    PubMed  Google Scholar 

  25. Rando RF, Srinivasan A, Feingold J, Gonczol E, Plotkin S: Characterization of multiple molecular interactions between human cytomegalovirus and human immunodeficiency virus type 1. Virology 1990, 176: 87–97.

    PubMed  Google Scholar 

  26. Pastore G, Santantonio T, Monno L, Milella M, Luchena N, Angarano G: Effects of HIV superinfection on HBV replication in a chronic HBsAg carrier with liver disease. Journal of Hepatology 1988, 7: 164–168.

    PubMed  Google Scholar 

  27. Bouffard P, Lamellin JP, Zoulim F, Pichoud C, Trepo C: Different form of hepatitis B virus DNA and expression of HBV antigens in peripheral blood mononuclear cells in chronic hepatitis B. Journal of Medical Virology 1990, 31: 312–317.

    PubMed  Google Scholar 

  28. Malavé C, Gorriño MT, Campelo C, Lardelli P, Cisterna R: Detection of hepatitis B virus DNA and determination of surface antigen expression in peripheral blood mononuclear cells from patients with AIDS. European Journal of Clinical Microbiology & Infectious Diseases 1994, 13: 267–271.

    Google Scholar 

  29. Hirschman SZ, Zucker ML: Recombinant DNA related to hepatitis B and human immunodeficiency viruses in mononuclear cells of patients with AIDS. Journal of Medical Virology 1988, 26: 145–152.

    PubMed  Google Scholar 

  30. Anderson MLM, Young BD: Quantitative filter hybridisation. In: Hames BD, Higgins SJ (ed): Nucleic acid hybridization. A practical approach. IRL Press, Washington, DC, 1987, p. 73–111.

    Google Scholar 

  31. Laurence J: Molecular interactions among herpesviruses and human immunodeficiency viruses. Journal of Infectious Diseases 1990, 162: 338–346.

    PubMed  Google Scholar 

  32. Nelson JA, Reynolds-Kohler C, Oldstone MBA, Willey CA: HIV and HCMV coinfect brain cells in patients with AIDS. Virology 1988, 165: 286–290.

    PubMed  Google Scholar 

  33. Casareale D, Fiala M, Chang CM, Cone LA, Mocarski ES: Cytomegalovirus enhances lysis of HIV-infected T lymphoblasts. International Journal of Cancer 1989, 44: 124–130.

    Google Scholar 

  34. Hadchouel M, Pasquinellli C, Fournier JG, Hugon RN, Scotto J, Bernard O, Brechot C: Detection of mononuclear cells expressing hepatitis B virus in peripheral blood from HBsAg positive and negative patients by in situ hybridisation. Journal of Medical Virology 1988, 24: 27–32.

    PubMed  Google Scholar 

  35. Pontisso P, Poon MC, Tiollais P, Brechot C: Detection of hepatitis B virus DNA in mononuclear blood cells. British Medical Journal 1984, 288: 1563–1566.

    PubMed  Google Scholar 

  36. Lie-Injo LE, Balasegaram M, Lopez CG, Herrera AR: Hepatitis B virus DNA in liver and white blood cells in patients with hepatoma. DNA 1983, 2: 301–308.

    PubMed  Google Scholar 

  37. Elfassi E, Romet-Lemonne JL, Essex M, Frances-McLane M, Haseltine WA: Evidence of extrachromosomal forms of hepatitis B viral DNA in a bone marrow culture obtained from a patient recently infected with hepatitis B virus. Proceedings of the National Academy of Sciences of the USA 1984, 81: 3526–3528.

    PubMed  Google Scholar 

  38. Laure F, Zagury D, Saimot AG, Gallo RC, Hahn B, Brechot C: Hepatitis B virus DNA sequence in lymphoid cells from patients with AIDS and AIDS related complex. Science 1985, 229: 561–563.

    PubMed  Google Scholar 

  39. Noonan CA, Loffe B, Mansell PWA, Melnick JL, Hollinger FB: Extrachromosomal sequences of hepatitis B virus DNA in peripheral blood mononuclear cells of acquired immunodeficiency syndrome patients. Proceedings of the National Academy of Sciences of the USA 1986, 83: 5698–5702.

    PubMed  Google Scholar 

  40. Chong-Jin O, Jenk-Ling B: Hepatitis B virus in lymphocytes of seronegative carriers. Lancet 1984, ii: 395–396.

    Google Scholar 

  41. Dalgleish AG, Beverley PCL, Clapham PR, Crawford DH, Greaves MF, Weiss RA: The CD4 (T4) antigen is an essential component of the receptor for the AIDS retrovirus. Nature 1984, 312: 763–767.

    PubMed  Google Scholar 

  42. Klatzman D, Champagne E, Chmaret S, Gruest J, Guetard D, Herrend T, Gluckman JC, Montagnier L: Tlymphocyte T4 molecule behaves as the receptor for human retrovirus LAV. Nature 1984, 312: 767–768.

    PubMed  Google Scholar 

  43. Maddon PJ, Dalgleish AG, McDougal JS, Clapham PR, Weiss RA, Axel R: The T4 gene encodes the AIDS virus receptor and is expressed in the immune system and the brain. Cell 1986, 47: 333–348.

    PubMed  Google Scholar 

  44. McDougal JS, Kennedy MS, Sligh JM, Cort SP, Mawle A, Nicholson JKA: Binding of HTLV-III/LAV to T4+ T cells by a complex of the 110K viral protein of the T4 molecule. Science 1986, 231: 382–385.

    PubMed  Google Scholar 

  45. Dahl K, Martin K, Miller G: Differences among human immunodeficiency virus strains in their capacities to induce cytolysis or persistent infection of a lymphoblastoid cell line inmortalized by Epstein-Barr virus. Journal of Virology 1987, 61: 1602–1608.

    PubMed  Google Scholar 

  46. Levy JA, Shimbukuro J, McHugh T, Casavant C, Stites D, Oshiro L: AIDS-associated retroviruses can productively infect other cells besides human helper T cells. Virology 1985, 147: 441–448.

    PubMed  Google Scholar 

  47. Monroe JE, Calender A, Mulder C: Epstein-Barr viruspositive and negative B-cell lines can be infected with human immunodeficiency virus types 1 and 2. Journal of Virology 1988, 62: 3497–3500.

    PubMed  Google Scholar 

  48. Montagnier L, Gruest J, Chamaret S, Dauget C, Aler C, Guetard D, Nugeyre F, Barre-Sinoussi F, Chermann JC, Brunet JB, Klatzmann D, Gluckman JC: Adaptation of lymphadenopathy associated virus to replication in EBV-transformed B lymphoblastoid cell lines. Science 1984, 225: 63–66.

    PubMed  Google Scholar 

  49. Salahuddin SZ, Ablashi DV, Hunter EA, Gonda MA, Sturzenegger S, Markham PD, Gallo RC: HTLV-III infection of EBV-genome positive B-lymphoid cells with or without detectable T4 antigens. International Journal of Cancer 1987, 39: 198–202.

    Google Scholar 

  50. Schrier RD, Nelson JA, Oldston MBA: Detection of human cytomegalovirus in peripheral blood lymphocytes in a natural infection. Science 1985, 230: 1048–1050.

    PubMed  Google Scholar 

  51. Sambucetti LC, Cherrington JM, Wilison GWG, Mocarski ES: NF-kB activation of the cytomegalovirus enhancer is mediated by a viral transactivator and by T cell stimulation. European Molecular Biology Organization Journal 1989, 8: 4251–4258.

    Google Scholar 

  52. Gaynor R: Cellular transcription factors involved in the regulation of HIV-1 gene expression. AIDS 1992, 6: 347–363.

    PubMed  Google Scholar 

  53. Diaz-Portillo J, Garcia-Martos P, Alvarez-Rojas C, Castaño-Lopez MA, Gonzalez-Borrachero ML, Martinez-Pais R: Infection by hepatitis B, C and delta viruses in carriers of human immunodeficiency virus. Medicina Clinica 1991, 96: 245–247.

    PubMed  Google Scholar 

  54. Levy JA: Pathogenesis of human immunodeficiency virus infection. Microbiological Reviews 1993, 57: 183–289.

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Immunology, Microbiology and Parasitology Department, University of Basque Country, PO Box 699, 48980, Bilbao, Spain

    R. Cisterna, C. Campelo, T. Gorriño, C. Malavé, L. Sarría, P. Lardelli, A. Fernandez de Aranguiz & L. Madariaga

  2. Microbiology Department, Civil Hospital of Bilbao, Bilbao, Spain

    R. Cisterna

Authors
  1. R. Cisterna
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. C. Campelo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. T. Gorriño
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. C. Malavé
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. L. Sarría
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. P. Lardelli
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. A. Fernandez de Aranguiz
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. L. Madariaga
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Cisterna, R., Campelo, C., Gorriño, T. et al. Association between HIV and other DNA viruses in vitro. Eur. J. Clin. Microbiol. Infect. Dis. 14, 591–596 (1995). https://doi.org/10.1007/BF01690730

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01690730

Keywords

Search

Navigation