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In vitro activity of omeprazole in combination with several antimicrobial agents against clinical isolates ofHelicobacter pylori

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Abstract

An agar dilution checkerboard method was used to evaluate the in vitro activity of omeprazole combined with clarithromycin, amoxicillin, and ceftibuten, respectively, against clinical isolates ofHelicobacter pylori. Mueller-Hinton agar plus 5% horse blood, an inoculum of 106 cfu/ml, and incubation of 72 h in a CO2 atmosphere were used. With the omeprazole and clarithromycin combination, synergism was observed in 51.5% and partial synergism in 39.3% of 33 strains; with omeprazole and amoxicillin, synergism was observed in 3% and partial synergism in 60.6% of 33 strains; and with omeprazole and ceftibuten, synergism was observed in 33.3% and partial synergism in 50% of 24 strains. Antagonism between omeprazole and each antibiotic was exhibited in 0%, 6.1%, and 4.1 % of these groups of strains, respectively. Of the antibiotic combinations tested, omeprazole plus clarithromycin exhibited synergism (partial or total) in the highest percentage of strains.

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Authors and Affiliations

  1. Department of Microbiology, Hospital Universitario de la Princesa, Diego de Leon 62, 28006, Madrid, Spain

    T. Alarcón, D. Domingo, I. Sánchez, F. Díaz de Rojas & M. López-Brea

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  1. T. Alarcón
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  2. D. Domingo
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  3. I. Sánchez
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  4. F. Díaz de Rojas
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  5. M. López-Brea
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Alarcón, T., Domingo, D., Sánchez, I. et al. In vitro activity of omeprazole in combination with several antimicrobial agents against clinical isolates ofHelicobacter pylori . Eur. J. Clin. Microbiol. Infect. Dis. 15, 937–940 (1996). https://doi.org/10.1007/BF01690512

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