Abstract
Protoplasts isolated from lateral shoot buds of cotyledon-free pea embryo axes were regenerated to callus. Protoplast derived calluses with a diameter of about 1cm were transferred to shoot induction media, containing different concentrations (1–50µM) of thidiazuron. Shoot formation was observed after 16 weeks up to 12% efficiency. Thidiazuron (10µM) was the most effective concentration in all experiments. Shoot buds elongated in medium supplemented with N-isopentenyl adenine and indole-3-butyric acid. Since rooting was almost impossible in these thidiazuron-induced shoots, shoots were grafted onto young pea seedlings and regenerated to fertile plants.
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Abbreviations
- 2ip:
-
N-isopentenyl adenine
- IAA:
-
indole-3-acetic acid
- IBA:
-
indole-3-butyric acid
- MES:
-
2-(Nmorpholino)ethane acid
- NAA:
-
naphthaleneacetic acid
- TDZ:
-
thidiazuron
- BAP:
-
6-benzylaminopurine
- PEG:
-
polyethylene glycol
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Böhmer, P., Meyer, B. & Jacobsen, HJ. Thidiazuron-induced high frequency of shoot induction and plant regeneration in protoplast derived pea callus. Plant Cell Reports 15, 26–29 (1995). https://doi.org/10.1007/BF01690247
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DOI: https://doi.org/10.1007/BF01690247