References
Borenfreund E, Puerner JA (1985) Toxicity determined in vitro by morphological alteration and neutral red absorption. Toxicol Lett 24:119–124
Borenfreund E, Puerner JA (1986) Cytotoxicity of metal, metal-metal, and metal-chelator combinations assayed in vitro. Toxicology 39:121–134
Chang MJW, Webb TE, Koestner A (1979) Distribution of O6-methylguanine in rat DNA following pretreatment in vivo with methylnitrosourea. Cancer Lett 6:123–127
Chilina AR, Chang M, Ives DH, Koestner A (1976) A new method for the isolation of eukaryotic nuclear proteins. Anal Biochem 72:552–565
Downs TR, Wilfinger WW (1983) Fluorometric quantification of DNA in cells and tissue. Anal Biochem 131:538–547
Hamilton-Koch W, Snyder RD, Lavelle JM (1986) Metal-induced DNA damage and repair in human diploid fibroblasts and Chinese hamster ovary cells. Chem-Biol Interact 59:17–28
Hutson TB, Mitchell ML, Keller JT, Long DJ, Chang MJW (1988) A technique for monitoring mammalian cell growth and inhibition in situ via Fourier transform infrared spectroscopy. Anal Biochem 174:415–422
Johnson-Wint B, Hollis S (1982) A rapid in situ deoxyribonucleic acid assay for determining cell number in culture and tissue. Anal Biochem 122:338–344
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275
Richards WL, Song MK, Krutzsch H, Evarts RP, Marsden E, Thorgeirsson SS (1985) Measurement of cell proliferation in microculture using Hoechst 33342 for the rapid semiautomated microfluorimetric determination of chromatin DNA. Exp Cell Res 159:235–246
Skehan P, Friedman SJ (1985) A rapid naphol yellow S method for measuring the cellular protein content of anchorage cultures. In vitro cell Develop Biol 21:288–290
Sorger T, Germinario RJ (1983) A direct solubilization procedure for the determination of DNA and protein in cultured fibroblast monolayers. Anal Biochem 131:254–256
West DC, Sattar A, Kumar S (1985) A simplified in situ solubilization procedure for the determination of DNA and cell number in tissue cultured mammalian cells. Anal Biochem 147:289–295
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Chang, M.J.W. UV absorption as an approximation for cell number inIn Vitro toxicity testing. Bull. Environ. Contam. Toxicol. 47, 190–197 (1991). https://doi.org/10.1007/BF01688639
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01688639