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Localization of a multi-catalytic, high-molecular mass proteinase in the nuclei of muscle cells

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Summary

A multi-catalytic protease in muscle cells was uniquely localized to the nucleus of muscle cells, both in cell culture and in sections of muscle tissue. Although no specific substructure of the nucleus could be identified as the site of the enzyme by immunocytochemical techniques, the enzyme was nevertheless present in all muscle cell nuclei. It appears that MCP is a useful marker for nuclei found specifically in muscle tissue.

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References

  • BARRETT, A. J. (1969) Properties of lysosomal enzymes. InLysosomes in Biology and Pathology (edited by DINGLE, J. T. & FELL, H. B.) Vol. 2, pp. 245–312. New York: John Wiley & Sons.

    Google Scholar 

  • BARRETT, A. J. (1980) The many forms and functions of cellular proteinases.Fedn. Proc. 39 9–14.

    Google Scholar 

  • BARRETT, A. J. & MCDONALD, J. K. (1980)Mammalian Proteases: A Glossary and Bibliography Vol. 1.Endopeptidases (edited by BARRETT, A. J. & McDONALD, J. K. p. 263. London: Academic.

    Google Scholar 

  • BEYNON, R. J., SHANNON, J. D. & BOND, J. S. (1981) Purification and characterization of a metalloendoproteinase from mouse kidney.Biochem. J. 199 591–8.

    PubMed  Google Scholar 

  • DAHLMANN, B., KUEHN, L. & REINAUER, H. (1983) Identification of three high molecular mass cysteine proteinases from rat skeletal muscle.FEBS Lett. 160 243–7.

    PubMed  Google Scholar 

  • DAHLMANN, B., KUEHN, L., RUTSCHMANN, M. & REINAUER, H. (1985a) Purification and characterization of a multicatalytic high-molecular-mass proteinase from rat skeletal muscle.Biochem. J. 228 161–70.

    PubMed  Google Scholar 

  • DAHLMANN, B., RUTSCHMANN, M., KUEHN, L., REINAUER, H. (1985b) Activation of the multicatalytic proteinase from rat skeletal muscle by fatty acids or sodium dodecyl sulfate.Biochem. J. 228 171–7.

    PubMed  Google Scholar 

  • DJONDJUROV, L. P., YANCHEVA, N. Y., IVANOVA, E. C. & CHRISTOV, K. (1984) Increased proteolysis in chromatin of terminally differentiated and quiescent cells.Expl. cell. Res. 152 134–47.

    Google Scholar 

  • FURLAN, M., JERICIJO, M. & SUHAR, A. (1968) Purification and properties of a neutral protease from calf thymus nuclei.Biochem. biophys. Acta 167 154–60.

    PubMed  Google Scholar 

  • HARE, J. F. (1978) A novel proteinase associated with mitochondrial membranes.Biochem. biophys. Res. Commun. 83 1206–15.

    PubMed  Google Scholar 

  • JACKSON, R. C. & BLOBEL, G. (1977) Post-translational cleavage of presecretory proteins with an extract of rough microsomes from dog pancreas containing signal peptidase activity.Proc. natn. Acad. Sci. U.S.A.,74, 926–33.

    Google Scholar 

  • KORANT, B. D. (1977) Protease activity associated with HeLa cell ribosomes.Biochem. biophys. Res. Commun. 74 926–33.

    PubMed  Google Scholar 

  • LOWRY, O. H., ROSENBROUGH, J. N., FARR, A. L. & RANDALL, R. J. (1951) Protein measurement with the folin phenol reagent.J. biol. Chem. 193 265–75.

    PubMed  Google Scholar 

  • MAYOREK, N., PINSON, A. & MAYER, M. (1979) Intracellular proteolysis in rat cardiac and skeletal muscle cells in culture.J. cell. Physiol. 98 587–96.

    PubMed  Google Scholar 

  • MCDONALD, J. K. (1985) An overview of protease specificity and catalytic mechanisms: aspects related to nomenclature and classification.Histochem. J. 17 773–85.

    PubMed  Google Scholar 

  • ROSE, I. A., WARMS, J. V. B. & HERSHKO, A. (1979) A high molecular weight protease in liver cytosol.J. biol. Chem. 254 8135–8.

    PubMed  Google Scholar 

  • STAUBER, W. T., FRITZ, V. K., DAHLMANN, B. & REINAUER, H. (1983) Immunohistochemical localization of two proteinases in skeletal muscle.J. Histochem. Cytochem. 31 827–30.

    PubMed  Google Scholar 

  • STAUBER, W. T., FRITZ, V. K., DAHLMANN, B. & REINAUER, H. (1986) Immunofluorescent localization of an alkaline proteinase in skeletal muscles from diabetic rats.Bas. appl. Histochem. 30 147–52.

    Google Scholar 

  • STAUBER, W. T., FRITZ, V. K., DAHLMANN, B., KAY, J., HEATH, R. & MAYER, M. (1987) Alkaline proteinase localization in myoblasts.J. Histochem. Cytochem. 35 83–6.

    PubMed  Google Scholar 

  • SZEGO, C. M. & PIETRAS, R. J. (1984) Lysosomal functions in cellular activation: Propagation of the actions of hormones and other effectors.Int. Rev. Cytol. 88 1–302.

    PubMed  Google Scholar 

  • YAMASHITA, N., KIHIRA, Y., MIYAZAKI, K., YAMASHITA, J. & HORIO, T. (1985) Dense distribution of nuclear alkaline protease to nucleoli with increase in rapidly growing cells in rats.Biochem. biosphys. Res. Commun. 132 1110–15.

    Google Scholar 

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Stauber, W.T., Fritz, V.K., Maltin, C.A. et al. Localization of a multi-catalytic, high-molecular mass proteinase in the nuclei of muscle cells. Histochem J 19, 594–597 (1987). https://doi.org/10.1007/BF01687368

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  • DOI: https://doi.org/10.1007/BF01687368

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