Summary
In order to demonstrate the localization of an anti-allergic agent, Tranilast, in the mast cells, light microscopic radioautography was performed. The mast cells collected from rat peritoneal cavity were incubated for 0 to 60 min in a medium containing3H-Tranilast. After the incubation, they were fixed, embedded and processed for light microscopic radioautography. The radioautographic silver grains were frequently localized around and over the cytoplasmic granules and their number increased according to the prolongation of incubation time. From the results obtained at present it was demonstrated that Tranilast was rapidly taken into the cytoplasm of mast cells. This phenomenon may suggest an important role of this agent in the inhibition of allergic reactions of mast cells.
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AZUMA, H., BANNO, K. & YOSHIMURA, Y. (1976) Pharmacological properties ofN-(3′,4′-dimethoxycinnamoyl)anthranilic acid (N-5′), a new anti-atopic agent.Brit. J. Pharmacol. 58, 483–8.
KARNOVSKY, M. J. (1965) A formaldehyde—glutaraldehyde fixative of high osmolality for use in electron microscopy.J. Cell Biol. 27, 137A–8A.
KODA, A., NAGAI, H., WATANABE, S., YANAGIHARA, Y & SAKAMOTO, K. (1976) Inhibition of hypersensitivity reactions by a new drug,N-(3′,4′-dimethoxycinnamoyl)anthranilic acid (N-5′).J. Allergy Clin. Immunol. 57, 396–407.
KODA, A., KURASHINA, Y. & NAKAZAWA, M. (1985) The inhibition mechanism of histamine release byN-(3,4-dimethoxycinnamoyl)anthranilic acid.Int. Archs Allergy appl. Immunol. 77, 244–5.
KUBOTA, T., UJIIE, A., NAITO, J., NAKAZAWA, M. & KODA, A. (1983) Studies on the mechanisms of the inhibitory effect ofN-5′ on histamine release from rat peritoneal exudate cells.Jpn J. Pharmacol. 33, 837–43.
LAGUNOFF, D. (1972) The mechanism of histamine release from mast cells.Biochem. Pharmacol. 21, 1889–96.
LUFT, J. H. (1961) Improvement in epoxy resin embedding methods.J. Biophys. Biochem. Cytol. 9, 409–14.
MOMOSE, Y., NISHIGAKI, T., NAITO, J. & NAGATA, T. (1985a) A radioautographic study of distribution of an anti-allergic agent, Tranilast, in rat liver.Acta Histochem. Cytochem. 18, 656.
MOMOSE, Y., NISHIGAKI, T., NAITO, J. & NAGATA, T. (1985b) Absorption of an anti-allergic agent, Tranilast, from rat alimentary tract: a radioautographic study.Acta. Histochem. Cytochem. 18, 656.
NAGATA, T., SHIBATA, O. & NAWA, T. (1967) Simplified methods for mass production of radioautograph (Japanese with English summary).Acta. Anat. Nippon 42, 162–6.
NISHIGAKI, T. & SHIGEMATSU, H. (1982) Electron microscopical studies on degranulation of mast cells in Type I allergic reactions (Japanese).Jpn J. Inflam. 2, 339–40.
TANIZAKI, Y., KOMAGOE, H., SUDO, M., OHTANI, J., TADA, S., TAKAHASHI, K. & KIMURA, I. (1986) Effect of Tranilast on45Ca uptake of rat peritoneal mast cells stimulated by antigen (Japanese with English summary).Jpn J. Allergology 35, 60–6.
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Nishigaki, T., Momose, Y. & Nagata, T. Light microscopic radioautographic study of the localization of an anti-allergic agent, Tranilast, in rat mast cells. Histochem J 19, 533–536 (1987). https://doi.org/10.1007/BF01687360
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DOI: https://doi.org/10.1007/BF01687360