Skip to main content
SpringerLink
Log in

Demonstration of dihydro-orotate dehydrogenase inNeurospora crassa hyphae with a cytochemical procedure

  • Papers
  • Published:
The Histochemical Journal Aims and scope Submit manuscript

Synopsis

Dihydro-orotate dehydrogenase activity was demonstrated in the hyphae of the fungusNeurospora crassa by a cytochemical technique. The activity was slightly stronger in the hyphal tips thus demonstrating the more intense metabolic activity in these parts of the mycelium. Several control reactions showed that the staining reaction was specific.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Cohen, R. B. (1962). Histochemical demonstration of a dihydroorotate dehydrogenase system in tissues.Lab. Invest. II, 531–5.

    Google Scholar 

  • Eakin, R. T. &Mitchell, H. K. (1969). A mitochondrial dihydroorotate oxidase system inNeurospora crassa.Archs Biochem. Biophys. 134, 160–71.

    Google Scholar 

  • Lieberman, I. &Kornberg, A. (1953). Enzymatic synthesis and breakdown of a pyrimidine orotic acid. I. Dihydro-orotic dehydrogenase.Biochim. biophys. Acta 13, 223.

    Google Scholar 

  • Reiss, J. (1967a). Der cytochemische Nachweis von Dehydrogenasen bei Pilzen. I. Untersuchungen und Ausblicke zur Methodik.Arch. Mikrobiol. 57, 285–306.

    PubMed  Google Scholar 

  • Reiss, J. (1967b). Der cytochemische Nachweis von Dehydrogenasen bei Pilzen. II. Nachweis einzelner Dehydrogenasen.Arch. Mikrobiol. 57, 307–34.

    PubMed  Google Scholar 

  • Reiss, J. (1967c). Der cytochemische Nachweis von Cytochromoxydase und Peroxydasen bei Pilzen.Histochemie 9, 281–99.

    PubMed  Google Scholar 

  • Reiss, J. (1969a). Cytochemischer Nachweis von Hydrolasen in Pilzzellen. I. Glycosidasen.Histochemie 18, 12–23.

    PubMed  Google Scholar 

  • Reiss, J. (1969b). Die Unbrauchbarkeit von Kaliumhexacyanoferrat (III) als Indikator zum intrazellulären Nachweis von Succinat-Dehydrogenase bei Pilzen.Annals Histochem. 14, 185–8.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Special Botany, University of Mainz, Mainz, Germany

    J. Reiss

Authors
  1. J. Reiss
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Reiss, J. Demonstration of dihydro-orotate dehydrogenase inNeurospora crassa hyphae with a cytochemical procedure. Histochem J 3, 23–25 (1971). https://doi.org/10.1007/BF01686503

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01686503

Keywords

Search

Navigation