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Modification of Michael's method for determination of serum acetyl choline esterase activity

Conclusion

  1. 1.

    Through the improvement of Michael's method to determine CHE activity, it became clear that non-enzymatic hydrolysis of acetylcholin in the incubation medium depends on pHi and pHf, and enzymatic hydrolysis depends on pHi.

  2. 2.

    Using corrections b and i, stable and precise values of CHE activity has been able to gained. Calculated corrections b and i are shown, former as correction of non-enzymatic hydrolysis, latter as one of enzymatic hydrolysis.

  3. 3.

    Titration of incubation medium with acetic acid brings us comparable values between the units ofpH/hr. and μ mole/ml/hr. or min.

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Suzuki, K., Tauchi, K. Modification of Michael's method for determination of serum acetyl choline esterase activity. Bull. Environ. Contam. Toxicol. 14, 674–680 (1975). https://doi.org/10.1007/BF01685241

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Keywords

  • Hydrolysis
  • Waste Water
  • Acetic Acid
  • Titration
  • Water Management