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The activity of Δ5- hydroxysteroid dehydrogenase enzyme in the interrenal tissue of rainbow trout (Salmo gairdneri Richardson) exposed to sublethal concentrations of zinc

Abstract

The effects of exposure of rainbow trout (Salmo gairdneri Richardson) to three sublethal concentrations of zinc (0.248, 0.528 and 1.14 ppm) on Δ5-3ß-hydroxysteroid dehydrogenase (Δ5-3ßHSDH) enzyme activity in the head kidney tissue was investigated. The activity of this enzyme was localized by the reduction of a tetrazolium salt to formazan at the site of activity. Using subjective methods to indicate the degree of activity and indirectly assess the degree of corticosteroid production, the zincexposed rainbow trout showed a greater degree of Δ5-3ßHSDH activity compared to the control fish. This increase in enzyme activity has been associated with the stimulation of the pituitary-interrenal axis by the noxious stress of zinc.

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References

  1. BAILLIE, A.H., M.M. FERGUSON and D. MCK. HART: Development in steroid histochemistry. London and New York: Academic Press (1966).

    Google Scholar 

  2. BENGTSSON, B.E.: Oikos25, 370 (1974).

    Article  CAS  Google Scholar 

  3. BUTLER, D.G.: American Zoologist13, 839 (1973).

    CAS  Google Scholar 

  4. CHESTNUT, C.W.: The pituitary gland of coho salmon (Oncor-hynchus kisutch W.) and its function in gonad maturation and thyroid activity. Ph.D. Thesis, Simon Fraser University.

  5. CRANDALL, C.A. and C.J. GOODNIGHT: Limnology and Oceanography7, 233 (1962).

    Article  Google Scholar 

  6. CRANDALL, C.A. and C.J. GOODNIGHT: Trans. Amer. Mic. Soc.82, 59 (1963).

    Article  Google Scholar 

  7. DONALDSON, E.M. and H.M. DYE: J. Fish. Res. Board Can.32, 533 (1975).

    Article  CAS  Google Scholar 

  8. HILL, C.W. and P.O. FROMM: Gen. Comp. Endocrinol.11, 69, (1968).

    Article  CAS  Google Scholar 

  9. IDLER, D.R.: Steroids in nonmammalian vertebrates. London and New York: Academic Press (1972).

    Google Scholar 

  10. SANGALANG, G.B. and H.C. Freeman: Biology of Reprod.11, 429 (1974).

    Article  CAS  Google Scholar 

  11. SILBERGELD, E.R.: Bull. Env. Contamin. & Toxicol.11, 20 (1974).

    Article  CAS  Google Scholar 

  12. SNIESZKO, S.F.: J. Fish. Biol.6, 197 (1974).

    Article  Google Scholar 

  13. SUBHEDAR, N. and P.D.P. RAO: Gen. Comp. Endocrinol.23, 403 (1974).

    Article  CAS  Google Scholar 

  14. TARAS, M.J., A.E. GREENBURG, R.D. HOAK and M.C. RAND: Standard methods for the examination of water and wastewater, 13th edition. American Public Health Association, American Water Works Association, and Water Pollution Control Federation (1971).

  15. WATSON, T.A.: The effects of sublethal concentrations of zinc on histological, histochemical, growth and blood parameters in rainbow trout (Salmo gairdneri Richardson). M.Sc. Thesis, University of Guelph, Guelph, Ontario, Canada.

  16. WATTENBURG, L.W.: J. Histochem. Cytochem.6, 226 (1958).

    Google Scholar 

  17. WIEBE, J.P.: Gen. Comp. Endocrinol.12, 256 (1969).

    Article  CAS  Google Scholar 

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Watson, T.A., McKeown, B.A. The activity of Δ5- hydroxysteroid dehydrogenase enzyme in the interrenal tissue of rainbow trout (Salmo gairdneri Richardson) exposed to sublethal concentrations of zinc. Bull. Environ. Contam. Toxicol. 16, 173–181 (1976). https://doi.org/10.1007/BF01685224

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Keywords

  • Rainbow Trout
  • Corticosterone
  • Nicotinamide Adenine Dinucleotide
  • Sublethal Concentration
  • Weight Observation