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The acetate kinase ofClostridum acetobutylicum strain P262

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Abstract

Clostridum acetobutylicum strain P262 fermented glucose, pyruvate, or lactate, and the butyrate production was substrate-dependent. Differences in butyrate yield could not be explained by changes in butyrate kinase activities, but the butyrate production was inversely related to acetate kinase activity. The acetate kinase had a pH optimum of 8.0, aK m for acetate of 160 mM, and ak cat of 16,800 min-1. The enyzme had a native molecular mass of 78 kDa; the size of 42 kDa on SDS-PAGE indicated that the acetate kinase of strain P262 was a homodimer.

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Abbreviations

Acetyl-P :

Acetyl-phosphate

MTT :

3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide

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Authors and Affiliations

  1. Agricultural Research Service, USDA, 14853, Ithaca, NY, USA

    James B. Russell

  2. Section of Microbiology, Cornell University, Wing Hall, 14853, Ithaca, NY, USA

    Francisco Diez-Gonzalez & James B. Russell

  3. Department of Agricultural and Biological Engineering, Cornell University, 14853, Ithaca, NY, USA

    Jean B. Hunter

Authors
  1. Francisco Diez-Gonzalez
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  2. James B. Russell
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  3. Jean B. Hunter
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Diez-Gonzalez, F., Russell, J.B. & Hunter, J.B. The acetate kinase ofClostridum acetobutylicum strain P262. Arch. Microbiol. 166, 418–420 (1996). https://doi.org/10.1007/BF01682990

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  • DOI: https://doi.org/10.1007/BF01682990

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