Summary
Some effects of two isomeric polycyclic aromatic hydrocarbons, anthracene and phenanthrene, on the fine structure and cytochemistry of digestive cells in the marine musselMytilus edulis have been investigated. The cytochemical results show that increasing concentrations of anthracene and phenanthrene have different effects on the acid labilization time for laten β-glucuronidase which is used to measure the stability of lysosomal membranes. At the ultrastructural level the limiting membranes of secondary lysosomes appear multilayered, with discontinuities and overlaps. Under the conditions of the experiment, only phenanthrene produces changes in this configuration. Both macroautophagic and microautophagic processes occur in the control and hydrocarbon treatments, and complementary data from other studies indicate that autophagic processes are enhanced by polycyclic aromatic hydrocarbons. Phenanthrene also causes proliferation of the smooth endoplasmic reticulum in the digestive cells, although cytochemical measurements of smooth endoplasmic reticulum-associated NADPH-ferrihemoprotein reductase show that anthracene stimulates activity over a greater range of concentrations than phenanthrene. The different effects of the two isomers is taken as evidence that the molecular configuration of the compound determines its reactivity with membranes and its subsequent effect on the physiology of the cells.
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ABERNETHY, S., BOBRA, A. M., SHIU, W. Y., WELLS, P. G. & MACKAY, D. (1986) Acute lethal toxicity of hydrocarbons and chlorinated hydrocarbons to two planktonic crustaceans: the key-role of organism—water partitioning.Aquatic Toxicol. 8, 163–74.
ALLISON, A. C. (1969) Lysosomes and cancer. InLysosomes in Biology and Pathology, Vol. 2 (edited by DINGLE, J. T. and FELL, H. B.) pp. 178–204. Amsterdam, New York, Oxford: Elsevier.
ALTMAN, F. P. (1972) Quantitative dehydrogenase histochemistry with special reference to the pentose shunt dehydrogenases.Progr. Histochem. Cytochem. 4, 225–73.
BITENSKY, L., BUTCHER, R. S. & CHAYEN, J. (1973) Quantitative cytochemistry in the study of lysosomal function. InLysosomes in Biology and Pathology, Vol. 3 (edited by DINGLE, J. T.) pp. 465–510. Amsterdam, New York, Oxford: Elsevier.
BUTCHER, R. G. & ALTMAN, F. P. (1973) Studies on the reduction of tetrazolium salts. II. The measurement of the half reduced and fully reduced formazans of neotetrazolium chloride in tissue sections.Histochemie 37, 351–63.
CARLES, D. (1984) Bioaccumulation des hydrocarbures parMytilus galloprovincialis (Lamarck). Etude qualitative et quantitative: relation avec les modifications cytologiques au niveau de la glande digestive. PhD Thesis, University of Marseille.
DONKIN, P. & EVANS, S. V. (1984) Application of steam distillation in the determination of petroleum hydrocarbons in water and musselsMytilus edulis from dosing experiments with crude oil.Anal. Chimica Acta 156, 207–19.
LIVINGSTONE, D. R., MOORE, M. N., LOWE, D. M., NASCI, C. & FARRAR, S. V. (1986) Responses of the cytochrome P450 mono-oxygenase system to diesel oil in the common mussel,Mytilus edulis L. and the periwinkleLittorina littorea L.Aquatic Toxicol. 7, 79–91.
MACKAY, D., BOBRA, A., SHIU, W. Y. & YALKOWSKY, S. H. (1980) Relationships between aqueous solubility and octanol—water partition coefficients.Chemosphere 9, 701–11.
MARZELLA, L., AHLBERG, J. & GLAUMANN, H. (1980)In vitro uptake of particles by lysosomes.Exp. Cell Res. 129, 460–6.
MOORE, M. N. (1976) Cytochemical demonstration of latency of lysosomal hydrolases in digestive cells of the common mussel,Mytilus edulis and changes induced by thermal stress.Cell. Tiss. Res. 175, 279–87.
MOORE, M. N. (1979) Cellular responses to polycyclic aromatic hydrocarbons and phenobarbital inMytilus edulis.Marine Environ. Res. 2, 255–63.
MOORE, M. N. (1985) Cellular responses to pollutants.Marine Poll. Bull. 16, 134–9.
MOORE, M. N. & CLARKE, K. R. (1982) Use of microstereology and quantitative cytochemistry to determine the effects of crude oil-derived aromatic hydrocarbons on lysosomal structure and function in a marine bivalve mollusc,Mytilus edulis.Histochem. J. 14, 713–18.
MOORE, M. N. & FARRAR, S. V. (1985) Effects of polynuclear aromatic hydrocarbons on lysosomal membranes in molluscs.Marine Environ. Res. 17, 222–5.
MOORE, M. N. & VIARENGO, A. (1987) Lysosomal membrane fragility and catabolism of cytosolic proteins: evidence for a direct relationship.Experientia 43, 320–3.
MOORE, M. N., LOWE, D. M. & FIETH, P. E. M. (1978) Lysosomal responses to experimentally injected anthracene in the digestive cells ofMytilus edulis.Marine Biol. 48, 297–302.
MOORE, M. N., WIDDOWS, J., CLEARY, J. J., PIPE, R. K., SALKELD, P. N., DONKIN, P., FARRAR, S. V., EVANS, S. V. & THOMSON, P. E. (1984) Responses of the musselMytilus edulis to copper and phenanthrene: interactive effects.Marine Environ. Res. 14, 167–83.
MOORE, M. N., LIVINGSTONE, D. R. & WIDDOWS, J. (1987) Hydrocarbons in marine molluscs, biological effects and ecological consequences. InHydrocarbon Metabolism by Aquatic Organisms, (edited by VARANASI, U.) Florida: CRC Press (in press).
MORTIMORE, G. E., HUTSON, N. J. & SURMACZ, C. A. (1983) Quantitative correlation between proteolysis and macro- and microautophagy in mouse hepatocytes during starvation and re-feeding.Proc. Natl. Acad. Sci. USA. 80, 2179–83.
NOTT, J. A., MOORE, M. N., MAVIN, L. J. & RYAN, K. P. (1985) The fine structure of lysosomal membranes and endoplasmic reticulum in the digestive cells ofMytilus edulis exposed to anthracene and phenanthrene.Marine Environ. Res. 17, 226–9.
OWEN, G. (1972) Lysosomes, peroxisomes and bivalves.Sci. Prog. Oxf. 60, 299–318.
OWEN, G. (1973) The fine structure and histochemistry of the digestive diverticula of the protobranchiate bivalveNucula sulcata.Proc. Roy. Soc. (B) 183, 249–64.
RUCKPAUL, K. & BERNHARDT, R. (1984) Biochemical aspects of the mono-oxygenase system in the endoplasmic reticulum of the mammalian liver. InCytochrome P 450:Structural and Functional Relationships, Biochemical and Physicochemical Aspects of Mixed Function Oxidases (edited by RUCKPAUL, K. and REIN, H.) pp. 9–57. Berlin: Akademie-Verlag.
RYAN, K. P. & PURSE, D. H. (1984) Rapid freezing: specimen supports and cold gas layers.J. Microsc. 136, RP5-RP6.
RYAN, K. P. & PURSE, D. H. (1985) Plunge-cooling of tissue blocks: determinants of cooling rates.J. Microsc. 140, 47–54.
VAN NOORDEN, C. F. J. & BUTCHER, R. G. (1986) A quantitative histochemical study of NADPH—ferrihemoprotein reductase activity.Histochem. J. 18, 364–70.
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Nott, J.A., Moore, M.N. Effects of polycyclic aromatic hydrocarbons on molluscan lysosomes and endoplasmic reticulum. Histochem J 19, 357–368 (1987). https://doi.org/10.1007/BF01680453
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DOI: https://doi.org/10.1007/BF01680453