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Ultracentrifugal fractionation of human erythrocytes with respect to cell age

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Summary

Human red cells, labelledin vivo with radioactive iron, were spun in an ultracentrifugal field and the fractions so obtained were analyzed for radioactivity and hemoglobin content. The data were analyzed in terms of the degree of separation obtained in each age group of cells. The data show that it is possible to obtain a rather good separation with respect to the youngest cells, but that there is a rather poor separation with respect to cells older than 10–20 days of age.

Zusammenfassung

Menschliche Erythrozyten, welche mit Radiocisen etikettiert waren, wurden in der Ultrazentrifuge getrennt und die erhaltenen Fraktionen auf Radioaktivität und Hämoglobingehalt untersucht. Die Ergebnisse wurden im Hinblick auf die Verteilung der Altersgruppen analysiert. Es zeigte sich, daß eine ziemlich gute Abtrennung der jungen Zellen möglich ist, während die Trennung von Erythrozyten, die älter als 10–20 Tage sind, ziemlich schlechte Resultate ergibt.

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Garby, L., Hjelm, M. Ultracentrifugal fractionation of human erythrocytes with respect to cell age. Blut 9, 284–291 (1963). https://doi.org/10.1007/BF01678992

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  • DOI: https://doi.org/10.1007/BF01678992

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