Chromosomal location of fertility restoring genes for ‘wild abortive’ cytoplasmic male sterility using primary trisomics in rice

Summary

Identification and location of fertility restoring genes facilitates their deployment in a hybrid breeding program involving cytoplasmic male sterility (CMS) system. The study aimed to locate fertility restorer genes of CMSWA system on specific chromosomes of rice using primary trisomics of IR36 (restorer), CMS (IR58025A) and maintainer (IR58025B) lines. Primary trisomic series (Triplo 1 to 12) was crossed as maternal parent with the maintainer line IR58025B. The selected trisomic and disomic F1 plants were testcrossed as male parents with the CMS line IR58025A. Plants in testcross families derived from disomic F1 plants (Group I crosses) were all diploid; however, in the testcross families derived from trisomic F1 plants (Group II crosses), some trisomic plants were observed. Diploid plants in all testcross families were analyzed for pollen fertility using 1% IKI stain. All testeross families from Group I crosses segregated in the ratio of 2 fertile: 1 partially fertile+partially sterile: 1 sterile plants indicating that fertility restoration was controlled by two independent dominant genes: one of the genes was stronger than the other. Testcross families from Group II crosses segregated in 2 fertile: 1 partially fertile+ partially sterile: 1 sterile plants in crosses involving Triplo 1, 4, 5, 6, 8, 9, 11 and 12, but families involving triplo 7 and triplo 10 showed significantly higher X2 values, indicating that the two fertility restorer genes were located on chromosome 7 and 10. Stronger restorer gene (Rf-WA-1) was located on chromosome 7 and weaker restorer gene (Rf-WA-2) was located on chromosome 10. These findings should facilitate tagging of these genes with molecular markers with the ultimate aim to practice marker-aided selection for fertility restoration ability.

This is a preview of subscription content, access via your institution.

References

  1. Bahl, P.N. & S.S. Maan, 1973. Chromosomal location of male fertility restorer genes in six lines of common wheat. Crop Sci. 13: 317–320.

    Google Scholar 

  2. Bharai, T.S., S.S. Bains, G.S. Sidhu & M.R. Gagneja. 1991. Genetics of fertility restoration of ‘wild abortive’ cytoplasmic male sterility in rice,Oryza sativa L. Euphytica 56: 199–203.

    Google Scholar 

  3. Govinda Raj, K. & S.S. Virmani, 1988. Genetics of fertility restoration of ‘WA’ type cytoplasmic male sterility in rice. Crop Sci. 28: 787–792.

    Google Scholar 

  4. Khush, G.S., R.J. Singh, A.C. Sur & A.L. Librojo, 1984. Primary trisomics of rice: Origin, morphology, cytology and use in linkage mapping. Genetics 107: 141–163.

    Google Scholar 

  5. Li, Y.C. & L.P. Yuan, 1986. Genetic analysis of fertility restoration in male sterile lines of rice. In: Rice Genetics, pp. 617–632. IRRI. P.O. Box 933, Manila, Philippines.

    Google Scholar 

  6. Lin, S.C. & L.P. Yuan, 1980. Hybrid rice breeding in China. pp. 35–51. In: Innovative approaches to rice breeding. IRRI, P.O. Box 933, Manila, Philippines.

    Google Scholar 

  7. Shinjyo, C., 1969. Cytoplasmic-genetic male sterility in cultivated rice,Oryza sativa L. II. The inheritance of male sterility. Japan J. Genetics 44: 149–156.

    Google Scholar 

  8. Shinjyo, C., 1975. Genetical studies of cytoplasmic male sterility and fertility restoration in rice,Oryza sativa L. Sci. Bull. Coll Agr Univ. Ryukyus 22: 1–57.

    Google Scholar 

  9. Shinjyo, C., R. Nishime & Y. Watanabe, 1974. Inheritance of fertility restoring gene Rfx and Rf in male sterile cytoplasm derived from vanety Lead rice. Japan J. Breed (Suppl. 1) 130–131 (in Japanese).

    Google Scholar 

  10. Shinyjo, C. & Y. Watanabe, 1977. Allelism test of 2 genes showing fertility restoring effect for male sterile cytoplasm of Lead rice and their inheritance. Japan J. Breed (Suppl. 2): 70–71.

    Google Scholar 

  11. Virmani, S.S. & C. Shinjyo, 1988. Current status of analysis and symbols for male sterile cytoplasms and fertility restorer genes. Rice Genet. Newsl. 5: 9–15.

    Google Scholar 

  12. Virmani, S.S., J.B. Young, H.P. Moon, I. Kumar & J.C. Flinn, 1991. Increasing rice yields through exploitation of heterosis. IRRI Research Paper Series Number 156. IRRI, P. Box 933, Manila, Philippines.

    Google Scholar 

  13. Virmani, S.S., M.N. Prasad & I. Kumar, 1993. Breaking yield barrier in rice through exploitation of heterosis. In: New Frontiers in Rice Research, Directorate of Rice Research. Hyderabad 500030, India, pp. 76–85.

    Google Scholar 

  14. Virmani, S.S. & W. Banghui, 1988. Development of CMS lines in hybrid rice breeding. In: Hybrid Rice, pp. 103–114, IRRI, P.O. Box 933, Manila, Philippines.

    Google Scholar 

  15. Young, J.B. & S.S. Virmani, 1984. Inheritance of fertility restoration in a rice cross. Rice Genetics Newsletter, 1: 102–103.

    Google Scholar 

  16. Zhou, T., 1983. Analysis of R genes in hybrid Indica rice of ‘WA’ type. Acta Agron. Sin. 9: 241–247.

    Google Scholar 

  17. Yoshimura, A., N. Iwata & T. Omura. 1982. Linkage analysis by reciprocal translocation method in rice plants (Oryza sativa L.). III. Marker genes located in Chromosomes 2, 3, 4 and 7. Japan J. Breed. 32: 323–332.

    Google Scholar 

Download references

Author information

Affiliations

Authors

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Bharaj, T.S., Virmani, S.S. & Khush, G.S. Chromosomal location of fertility restoring genes for ‘wild abortive’ cytoplasmic male sterility using primary trisomics in rice. Euphytica 83, 169–173 (1995). https://doi.org/10.1007/BF01678126

Download citation

Key words

  • rice
  • Oryza sativa
  • cytoplasmic male sterility
  • fertility restoration
  • chromosomal gene location
  • primary trisomics
  • rice