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Genotypic variation and polymorphism of 2S albumins of sunflower

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Summary

2S albumin fractions from seeds of seven inbred lines and two cultivars of sunflower were compared using a combination of electrophoresis (one and two dimensional) and reversed-phase high performance liquid chromatography. Eleven to thirteen major components were present in each genotype with variation in their proportions and absence or presence. All appeared to be single chain proteins with relative molecular masser (M r ) between about 10.000 and 18.000; some had intra-chain disulphide bonds. Preliminary analyses of single F2 seeds indicated that some components were co-inherited, which could result from their being encoded by linked genes or by a single transcript from one gene.

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Abbreviations

cDNA:

cloned deoxyribonucleic acid

M r :

relative molecular mass

S 20.w :

sedimentation coefficient

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Author notes

  1. Irina N. Anisimova

    Present address: N. I. Vavilov Institute of Plant Industry, 44 Bolshaya Morskaya, 190000, St. Petersburg, Russia

Authors and Affiliations

  1. IACR Long Ashton Research Station, Department of Agricultural Sciences, University of Bristol, Long Ashton, BS18 9AF, Bristol, UK

    Irina N. Anisimova, Roger J. Fido, Arthur S. Tatham & Peter R. Shewry

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  1. Irina N. Anisimova
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  2. Roger J. Fido
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  3. Arthur S. Tatham
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  4. Peter R. Shewry
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Anisimova, I.N., Fido, R.J., Tatham, A.S. et al. Genotypic variation and polymorphism of 2S albumins of sunflower. Euphytica 83, 15–23 (1995). https://doi.org/10.1007/BF01677856

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  • DOI: https://doi.org/10.1007/BF01677856

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